Chen Yong, Liu Yuyan, Wang Liang, Li Heng, Linghu Tingting, Chen Yixin, Tian Houjun, Lin Shuo, Zheng Xue, Wei Hui
Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou Scientific Observing and Experimental Station of Crop Pests of Ministry of Agriculture, Fujian Engineering Research Center for Green Pest Management, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Microbiol. 2022 Jun 28;13:911751. doi: 10.3389/fmicb.2022.911751. eCollection 2022.
Tomato zonate spot virus (TZSV) is a phytopathogen of the genus () that is widespread in many areas of Southwest China. TZSV is mainly transmitted by , but its exact infection route remains unclear. To explore this issue, we detected the nucleocapsid protein of TZSV in the digestive systems of first-instar nymphs fed with TZSV-infected pepper leaves. TZSV infection in the digestive system begins within 4 h post-first access to diseased plants: The foregut is likely the primary site of infection, and primary salivary glands (PSGs) are the destination. There are three potential routes for TZSV transmission from the alimentary canal to the PSGs: (1) virus dissemination from the midgut to hemocoel followed by movement to the PSGs; (2) accumulation in midgut epithelial cells and arrival at PSGs tubular salivary glands and efferent ducts; and (3) arrival at epitheliomuscular cells of the forepart of the midgut and movement along the ligament to the PSGs. We tested the transmission efficiency of in second-instar nymphs and female and male adults. TZSV was transmitted in a persistent-propagative mode by both nymphs and adults, with adults appearing to show slightly higher transmission efficiency than nymphs. We confirmed the presence of all three routes for TZSV transmission in and determined that like other , TZSV is transmitted in a persistent-propagative manner. These results should facilitate the control of TZSV-related diseases and further our understanding of the transmission biology of in general.
番茄环斑病毒（TZSV）是（）属的一种植物病原体，在中国西南部的许多地区广泛分布。TZSV主要通过（）传播，但其确切的感染途径仍不清楚。为了探究这个问题，我们在喂食了感染TZSV的辣椒叶片的一龄若虫的消化系统中检测了TZSV的核衣壳蛋白。在首次接触患病植物后4小时内，TZSV在（）的消化系统中开始感染：前肠可能是主要感染部位，而初级唾液腺（PSG）是感染终点。从消化道到PSG，TZSV有三种潜在的传播途径：（1）病毒从中肠扩散到血腔，然后转移到PSG；（2）在中肠上皮细胞中积累，然后到达PSG、管状唾液腺和输出导管；（3）到达中肠前部的上皮肌细胞，并沿着韧带移动到PSG。我们测试了二龄若虫以及雌、雄成虫的（）传播效率。TZSV通过若虫和成虫以持久性增殖模式传播，成虫的传播效率似乎略高于若虫。我们证实了在（）中存在TZSV传播的所有三种途径，并确定与其他（）一样，TZSV以持久性增殖方式传播。这些结果应有助于控制与TZSV相关的疾病，并增进我们对一般（）传播生物学的理解。
