仙人掌籽油的化学成分：酚类物质鉴定及抗氧化活性
Chemical Composition of Cactus Pear Seed Oil: phenolics identification and antioxidant activity.
作者信息
Ali Berraaouan, Abderrahim Ziyyat, Hassane Mekhfi, Marianne Sindic, Marie-Laure Fauconnier, Abdelkhaleq Legssyer, Mohammed Aziz, Mohamed Bnouham
机构信息
Laboratory of Bioressources, Biotechnologies, Ethnopharmacology and Health, Mohammed the First University, Faculty of Sciences, Oujda, Morocco.
Laboratory of Agroalimentary Products Quality and Safety, Unit of Quality Analysis and Hazard, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
出版信息
J Pharmacopuncture. 2022 Jun 30;25(2):121-129. doi: 10.3831/KPI.2022.25.2.121.
OBJECTIVES
The chemical composition of cactus pear seed oil (Opuntia ficus-indica [L.] Mill.) was analyzed in terms of its fatty acid composition, tocopherol content, phenolic identification, and the oil's phenolic-rich fraction antioxidant power was determined.
METHODS
Fatty acid profiling was performed by gas chromatography coupled to an FI detector. Tocopherols and phenolic compounds were analyzed by LC-FLD/UV, and the oil's phenolic-rich fraction antioxidant power was determined by phosphomolybdenum, DPPH assay and β-carotene bleaching test.
RESULTS
Fatty acid composition was marked by a high unsaturation level (83.22 ± 0.34%). The predominant fatty acid was linoleic acid (66.79 ± 0.78%), followed by oleic acid (15.16 ± 0.42%) and palmitic acid (12.70 ± 0.03%). The main tocopherol was γ-tocopherol (172.59 ± 7.59 mg/kg. In addition, Tyrosol, vanillic acid, vanillin, ferulic acid, pinoresinol, and cinnamic acid were identified as phenolic compounds in the analyzed seed oil. Moreover, the oil's phenolics-rich fraction showed a significant total antioxidant activity, scavenged DPPH up to 97.85%, and effectively protected β-carotene against bleaching (97.56%).
CONCLUSION
The results support the potential use of cactus pear seed oil as a functional food.
目的
从仙人掌籽油（仙人掌[L.] Mill.）的脂肪酸组成、生育酚含量、酚类物质鉴定方面分析其化学成分，并测定该富含酚类物质的油馏分的抗氧化能力。
方法
采用气相色谱 - 火焰离子化检测器进行脂肪酸谱分析。生育酚和酚类化合物通过液相色谱 - 荧光检测器/紫外检测器进行分析，通过磷钼酸法、DPPH 法和β - 胡萝卜素漂白试验测定该富含酚类物质的油馏分的抗氧化能力。
结果
脂肪酸组成的特点是不饱和程度高（83.22 ± 0.34%）。主要脂肪酸为亚油酸（66.79 ± 0.78%），其次是油酸（15.16 ± 0.42%）和棕榈酸（12.70 ± 0.03%）。主要生育酚为γ - 生育酚（172.59 ± 7.59 mg/kg）。此外，在分析的种子油中鉴定出酪醇、香草酸、香草醛、阿魏酸、松脂醇和肉桂酸为酚类化合物。而且，该油的富含酚类物质的馏分表现出显著的总抗氧化活性，清除 DPPH 高达 97.85%，并有效保护β - 胡萝卜素不被漂白（97.56%）。
结论
结果支持仙人掌籽油作为功能性食品的潜在用途。
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