Department of Medical Laboratory Science, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.
Kombolcha 03 Health Center, Kombolcha, South Wollo, Amhara Regional State, Ethiopia.
PLoS One. 2022 Jul 15;17(7):e0270685. doi: 10.1371/journal.pone.0270685. eCollection 2022.
Hematological reference intervals (RIs) are affected by inherent variables like age, sex, genetic background, environment, diet and certain circumstances such as pregnancy signifying the need for population specific values. This study was designed to establish RIs for common hematological parameters of apparently healthy pregnant and non-pregnant women from Northeast Ethiopia.
This community based cross-sectional study recruited 600 pregnant and non-pregnant women in South Wollo Zone, Northeast Ethiopia from June to August 2019. Complete blood count was performed for eligible participants using Mindary BC-3000 plus hematology analyzer. The mean, median, and 2.5th and 97.5th percentile reference limits with 90% CI were determined using SPSS version 23.
The established selected 2.5th-97.5th percentiles RIs for pregnant women were: WBC: 4.0-13.2x109/L; RBC: 3.45-4.67x1012/L; Hgb: 10.1-13.7g/dL; HCT: 33.5-46.5%; MCV: 85-104fL; MCH: 27.5-33.0pg; MCHC: 30.3-33.7g/dL and Platelet count: 132-373x109/L. The respective values for non-pregnant women were 3.6-10.3; 4.44-5.01; 12.4-14.3; 38.4-50.1; 86-102; 27.1-32.4, 30.4-34.1, 173-456. A statistically significant difference between pregnant and non-pregnant women was noted in all hematological parameters except MCHC. The mean and median value of WBC count, MCV, MPV and PDW increased, whereas mean values of HCT and Platelet count decreased as gestational period advances.
The observed difference from other studies signify the necessity for using trimester specific RIs and separate RI for pregnant and non-pregnant women. Thus, we recommend the health facilities in the study area to utilize the currently established RIs for pregnant and non-pregnant women for better care.
血液学参考区间（RIs）受内在变量的影响，如年龄、性别、遗传背景、环境、饮食以及妊娠等特殊情况，这表明需要建立特定人群的参考值。本研究旨在建立来自埃塞俄比亚东北沃洛地区的健康孕妇和非孕妇的常见血液学参数的 RIs。
本社区横断面研究于 2019 年 6 月至 8 月在埃塞俄比亚东北南沃洛地区招募了 600 名孕妇和非孕妇。使用 Mindary BC-3000 加血液学分析仪对合格的参与者进行全血细胞计数。使用 SPSS 版本 23 确定平均值、中位数以及 2.5%和 97.5%参考限值和 90%CI。
确定的孕妇选定的 2.5%至 97.5%的 RI 为：WBC：4.0-13.2x109/L；RBC：3.45-4.67x1012/L；Hgb：10.1-13.7g/dL；HCT：33.5-46.5%；MCV：85-104fL；MCH：27.5-33.0pg；MCHC：30.3-33.7g/dL 和血小板计数：132-373x109/L。非孕妇的相应值分别为 3.6-10.3；4.44-5.01；12.4-14.3；38.4-50.1；86-102；27.1-32.4；30.4-34.1；173-456。所有血液学参数中，除 MCHC 外，孕妇和非孕妇之间均存在统计学显著差异。WBC 计数、MCV、MPV 和 PDW 的平均值和中位数增加，而 HCT 和血小板计数的平均值随着妊娠周期的进展而降低。
与其他研究的观察结果存在差异表明，有必要使用特定妊娠阶段的 RI 和单独的孕妇和非孕妇 RI。因此，我们建议研究区域的医疗机构利用目前为孕妇和非孕妇建立的 RI，以提供更好的护理。
