Bourque J E, Miller J C, Park W D
In Vitro Cell Dev Biol. 1987 May;23(5):381-6. doi: 10.1007/BF02620996.
Nodal cuttings from micropropagated potato plantlets give rise to microtubers when placed on Murashige and Skoog medium containing 6% sucrose and 2.5 mg/liter kinetin and incubated in the dark at 19 degrees C. Microtubers produced from the cultivar Superior were shown to contain the same characteristic group of proteins as field-grown tubers. As with field-grown tubers, the 40,000-dalton major tuber glycoprotein, patatin, accumulated to high levels in microtubers, reaching 3.7 +/- 0.2 mg/g fresh weight after 90 d. Also in agreement with field-grown plants, stems and leaves of micropropagated plantlets did not contain detectable levels of patatin, but small amounts of an electrophoretically distinct form accumulated transiently in roots. Patatin mRNA is readily detectable in developing microtubers 15 d after transfer of the cuttings to inductive medium. Patatin mRNA was also present in roots, but as with field-grown plants, was 50- to 100-fold less abundant and could be distinguished from that in tubers by primer extension. Microtuber development and patatin accumulation were inhibited by gibberellic acid.
将微繁殖马铃薯苗的节段切下，置于含有6%蔗糖和2.5毫克/升激动素的Murashige和Skoog培养基上，并在19℃黑暗条件下培养时，会形成微型块茎。从“Superior”品种产生的微型块茎显示含有与田间种植块茎相同的特征性蛋白质组。与田间种植的块茎一样，40000道尔顿的主要块茎糖蛋白——马铃薯Patatin蛋白，在微型块茎中大量积累，90天后达到3.7±0.2毫克/克鲜重。同样与田间种植的植株一致，微繁殖苗的茎和叶中未检测到Patatin蛋白，但根中短暂积累了少量电泳上不同的形式。在将插条转移到诱导培养基15天后，发育中的微型块茎中很容易检测到Patatin mRNA。根中也存在Patatin mRNA，但与田间种植的植株一样，其丰度低50至100倍，并且通过引物延伸可与块茎中的Patatin mRNA区分开来。赤霉素抑制微型块茎的发育和Patatin蛋白的积累。
