Mignery G A, Pikaard C S, Hannapel D J, Park W D
Nucleic Acids Res. 1984 Nov 12;12(21):7987-8000. doi: 10.1093/nar/12.21.7987.
A cDNA library from membrane-bound poly(A)+ mRNA of developing potato tubers was constructed and two classes of essentially full-length cDNA clones for a precursor to the major tuber glycoprotein, patatin were isolated. Sequence analysis shows that the two classes are approximately 99% homologous and correspond to the two major species of patatin identified previously by NH2-terminal amino acid sequence analysis. Sequence analysis also predicts that patatin is synthesized with a 23 amino acid signal sequence. Northern blot analysis shows that patatin mRNAs are 1550 +/- 50 nucleotides in length and are normally not present in polyribosomal or total RNA from stems or leaves.
构建了来自发育中马铃薯块茎膜结合多聚腺苷酸（poly(A)+）mRNA的cDNA文库，并分离出了两类主要块茎糖蛋白（即马铃薯块茎蛋白）前体的基本全长cDNA克隆。序列分析表明，这两类克隆的同源性约为99%，对应于先前通过氨基末端氨基酸序列分析鉴定出的两种主要马铃薯块茎蛋白。序列分析还预测，马铃薯块茎蛋白是由一个23个氨基酸的信号序列合成的。Northern印迹分析表明，马铃薯块茎蛋白mRNA的长度为1550±50个核苷酸，通常不存在于茎或叶的多核糖体或总RNA中。
