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丁香假单胞菌recA基因的分子克隆与生物学特性分析

Molecular cloning and biological characterization of the recA gene from Pseudomonas syringae.

作者信息

Hickman M J, Orser C S, Willis D K, Lindow S E, Panopoulos N J

出版信息

J Bacteriol. 1987 Jun;169(6):2906-10. doi: 10.1128/jb.169.6.2906-2910.1987.

DOI:10.1128/jb.169.6.2906-2910.1987
PMID:3584077
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212212/
Abstract

We have identified a recombinant plasmid, pCUV8, from a cosmid library of Pseudomonas syringae genomic DNA which contains a functional analog of the Escherichia coli recA gene. The plasmid was initially identified by its ability to restore UV resistance to E. coli HB101. Quantitative analysis demonstrated that it restored both recombination proficiency and UV resistance to an E. coli recA deletion mutant. By these criteria, pCUV8 appears to contain the P. syringae recA gene. Several pathogenic and epiphytic strains of P. syringae, but not E. coli, showed sequence homology to pCUV8 under normal stringency.

摘要

我们从丁香假单胞菌基因组DNA的黏粒文库中鉴定出一种重组质粒pCUV8，它含有大肠杆菌recA基因的功能类似物。该质粒最初是因其能恢复大肠杆菌HB101对紫外线的抗性而被鉴定出来的。定量分析表明，它能恢复大肠杆菌recA缺失突变体的重组能力和对紫外线的抗性。根据这些标准，pCUV8似乎含有丁香假单胞菌的recA基因。在正常严谨度下，几种丁香假单胞菌的致病菌株和附生菌株（而非大肠杆菌）与pCUV8显示出序列同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/634c/212212/eb8779bfe567/jbacter00196-0596-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/634c/212212/eb8779bfe567/jbacter00196-0596-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/634c/212212/eb8779bfe567/jbacter00196-0596-a.jpg

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