Department of Microbiology, Techno India University, EM-4 Sector-V, Salt Lake City, Kolkata, West Bengal 700091, India.
Department of Biotechnology, University of Calcutta, West Bengal, India.
Colloids Surf B Biointerfaces. 2022 Sep;217:112688. doi: 10.1016/j.colsurfb.2022.112688. Epub 2022 Jul 8.
Worldwide, the emergence of diarrhoea-causing multi-drug resistant (MDR) bacteria has become a crucial problem in everyday life. Tetracycline (TC) is a bacteriostatic agent that has a wide spectrum of antibacterial activity. One potential strategy to enhance the penetration and antibacterial activity of antibiotics is the use of nanotechnology. In this context, this study dealt with the synthesis of TC loading in biocompatible magnesium oxide nanoparticles (MgONPs), its characterization, and the potency of killing against diarrhoea-causing MDR bacteria E. coli and S. flexneri. TC loaded- MgONPs (MgONPs-TC) were characterized by DLS, SEM-EDS, UV-vis spectroscopy, and FTIR techniques with adequate physical properties. Antibacterial and antibiofilm studies indicate that this nanoparticle successfully eradicated both planktonic and sessile forms of those bacteria. It also significantly reduced the production of bacterial EPS, different levels of antioxidant enzymes, and induced reactive oxygen species (ROS) in the bacterial cell as a mode of antibacterial action. In particular, MgONPs-TC were efficient in reducing the colonization of MDR E. coli and S. flexneri in the C. elegans model. Therefore, all these data suggest that MgONPs-TC are a highly promising approach to combating diseases associated with diarrhoea-causing MDR bacteria in the medical field with limited health care budgets.
在全球范围内，导致腹泻的多药耐药（MDR）细菌的出现已成为日常生活中的一个关键问题。四环素（TC）是一种抑菌剂，具有广泛的抗菌活性。一种增强抗生素穿透性和抗菌活性的潜在策略是利用纳米技术。在这种情况下，本研究涉及将 TC 负载于生物相容性的氧化镁纳米粒子（MgONPs）中的合成、表征，以及针对引起腹泻的 MDR 细菌大肠杆菌和志贺氏菌的杀菌效力。TC 负载的 MgONPs（MgONPs-TC）通过 DLS、SEM-EDS、UV-vis 光谱和 FTIR 技术进行了表征，具有适当的物理性质。抗菌和抗生物膜研究表明，这种纳米颗粒成功地根除了这两种细菌的浮游和固着形式。它还显著降低了细菌 EPS 的产生、不同水平的抗氧化酶，并诱导了细菌细胞中的活性氧物质（ROS），作为其抗菌作用的一种方式。特别是，MgONPs-TC 能够有效地减少 MDR 大肠杆菌和志贺氏菌在秀丽隐杆线虫模型中的定植。因此，所有这些数据表明，MgONPs-TC 是一种很有前途的方法，可以在医疗领域对抗与引起腹泻的 MDR 细菌相关的疾病，特别是在医疗保健预算有限的情况下。
