Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Department of Chemistry and Biotechnology, Tallinn University of Technology (TalTech), Akadeemia tee 15, Tallinn 12618, Estonia.
Curr Opin Biotechnol. 2022 Aug;76:102755. doi: 10.1016/j.copbio.2022.102755. Epub 2022 Jul 13.
Application of droplet-based methods enables (i) faster detection, (ii) increased sensitivity, (iii) characterization of the level of heterogeneity in response to antibiotics by bacterial populations, and (iv) expanded screening of the effectiveness of antibiotic combinations. Hereby, we discuss the key steps and parameters of droplet-based experiments to investigate antimicrobial resistance. We also review recent findings accomplished with these methods and highlight their advantages and capacity to yield new insights into the problem of antimicrobial resistance.
基于液滴的方法的应用使得(i)更快的检测,(ii)更高的灵敏度,(iii)对细菌群体对抗生素反应的异质性水平进行特征分析,以及(iv)扩大了抗生素组合效果的筛选。在此,我们讨论了基于液滴的实验来研究抗微生物药物耐药性的关键步骤和参数。我们还回顾了这些方法最近取得的发现,并强调了它们的优势及其产生对抗微生物药物耐药性问题的新见解的能力。