在自由活动的小鼠中进行长期 SCN 钙信号记录。

Long-term SCN calcium signal recording in freely moving mice.

机构信息

Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Su Genomic Resource Center, Medical School of Soochow University, Suzhou, Jiangsu 215123, China; Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Su Genomic Resource Center, Medical School of Soochow University, Suzhou, Jiangsu 215123, China.

出版信息

STAR Protoc. 2022 Sep 16;3(3):101547. doi: 10.1016/j.xpro.2022.101547. Epub 2022 Jul 16.

DOI:10.1016/j.xpro.2022.101547

PMID:35842865

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9294265/

Abstract

The suprachiasmatic nucleus (SCN) is the master circadian pacemaker of the mammalian biological clock. Here, we provide a detailed protocol for long-term recording of calcium signals in SCN neurons of freely moving mice through a multichannel optical fiber recording system. This system can simultaneously collect calcium signals from up to seven animals. The calcium signals can be visualized by the appropriate software and code. This protocol can be used to explore the long-term response of SCN to external environmental stimulation. For complete details on the use and execution of this protocol, please refer to Zhai et al. (2022).

摘要

视交叉上核（SCN）是哺乳动物生物钟的主生物钟起搏器。在这里，我们提供了一个详细的方案，通过多通道光纤记录系统对自由活动的小鼠 SCN 神经元中的钙信号进行长期记录。该系统可以同时从多达七个动物中收集钙信号。钙信号可以通过适当的软件和代码进行可视化。该方案可用于探索 SCN 对外部环境刺激的长期反应。有关此方案的使用和执行的完整详细信息，请参阅 Zhai 等人。（2022 年）。