Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, 100871 Beijing, China.
National Institute of Biological Sciences, 102206 Beijing, China.
Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):4276-4281. doi: 10.1073/pnas.1717735115. Epub 2018 Apr 2.
Endogenous circadian clocks control 24-h physiological and behavioral rhythms in mammals. Here, we report a real-time in vivo fluorescence recording system that enables long-term monitoring of circadian rhythms in the brains of freely moving mice. With a designed reporter of circadian clock gene expression, we tracked robust transcription reporter rhythms in the suprachiasmatic nucleus (SCN) of WT, , and mice in LD (12 h light, 12 h dark) and DD (constant darkness) conditions and verified that signals remained stable for over 6 mo. Further, we recorded transcriptional rhythms in the subparaventricular zone (SPZ) and hippocampal CA1/2 regions of WT mice housed under LD and DD conditions. By using a Cre-loxP system, we recorded and transcription rhythms specifically in vasoactive intestinal peptide (VIP) neurons of the SCN. Finally, we demonstrated the dynamics of and transcriptional rhythms in SCN VIP neurons following an 8-h phase advance in the light/dark cycle.
内源性生物钟控制着哺乳动物 24 小时的生理和行为节律。在这里，我们报告了一种实时活体荧光记录系统，该系统能够长期监测自由活动小鼠大脑中的生物钟节律。利用生物钟基因表达的设计报告基因，我们在 WT、 和 小鼠的视交叉上核（SCN）中跟踪了强大的转录报告基因节律，在 LD（12 小时光照，12 小时黑暗）和 DD（持续黑暗）条件下，并验证了信号在超过 6 个月的时间内保持稳定。此外，我们记录了 WT 小鼠在 LD 和 DD 条件下下丘核（SPZ）和海马 CA1/2 区的 转录节律。通过使用 Cre-loxP 系统，我们专门记录了 SCN 中血管活性肠肽（VIP）神经元中的 和 转录节律。最后，我们展示了在光/暗周期中 8 小时提前光照后 SCN VIP 神经元中 和 转录节律的动态变化。
