在自由活动的小鼠大脑中进行长期的体内生物钟节律记录。
Long-term in vivo recording of circadian rhythms in brains of freely moving mice.
机构信息
Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, 100871 Beijing, China.
National Institute of Biological Sciences, 102206 Beijing, China.
出版信息
Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):4276-4281. doi: 10.1073/pnas.1717735115. Epub 2018 Apr 2.
Abstract
Endogenous circadian clocks control 24-h physiological and behavioral rhythms in mammals. Here, we report a real-time in vivo fluorescence recording system that enables long-term monitoring of circadian rhythms in the brains of freely moving mice. With a designed reporter of circadian clock gene expression, we tracked robust transcription reporter rhythms in the suprachiasmatic nucleus (SCN) of WT, , and mice in LD (12 h light, 12 h dark) and DD (constant darkness) conditions and verified that signals remained stable for over 6 mo. Further, we recorded transcriptional rhythms in the subparaventricular zone (SPZ) and hippocampal CA1/2 regions of WT mice housed under LD and DD conditions. By using a Cre-loxP system, we recorded and transcription rhythms specifically in vasoactive intestinal peptide (VIP) neurons of the SCN. Finally, we demonstrated the dynamics of and transcriptional rhythms in SCN VIP neurons following an 8-h phase advance in the light/dark cycle.
摘要
内源性生物钟控制着哺乳动物 24 小时的生理和行为节律。在这里，我们报告了一种实时活体荧光记录系统，该系统能够长期监测自由活动小鼠大脑中的生物钟节律。利用生物钟基因表达的设计报告基因，我们在 WT、 和 小鼠的视交叉上核（SCN）中跟踪了强大的转录报告基因节律，在 LD（12 小时光照，12 小时黑暗）和 DD（持续黑暗）条件下，并验证了信号在超过 6 个月的时间内保持稳定。此外，我们记录了 WT 小鼠在 LD 和 DD 条件下下丘核（SPZ）和海马 CA1/2 区的 转录节律。通过使用 Cre-loxP 系统，我们专门记录了 SCN 中血管活性肠肽（VIP）神经元中的 和 转录节律。最后，我们展示了在光/暗周期中 8 小时提前光照后 SCN VIP 神经元中 和 转录节律的动态变化。
相似文献
1
Long-term in vivo recording of circadian rhythms in brains of freely moving mice.在自由活动的小鼠大脑中进行长期的体内生物钟节律记录。
Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):4276-4281. doi: 10.1073/pnas.1717735115. Epub 2018 Apr 2.
2
Advanced light-entrained activity onsets and restored free-running suprachiasmatic nucleus circadian rhythms in per2/dec mutant mice.节律基因 Per2/Dec 突变小鼠光控活动起始和恢复自由运行视交叉上核节律。
Chronobiol Int. 2011 Nov;28(9):737-50. doi: 10.3109/07420528.2011.607374.
3
Postnatal constant light compensates Cryptochrome1 and 2 double deficiency for disruption of circadian behavioral rhythms in mice under constant dark.在持续黑暗环境下，光暗循环中生物钟行为节律紊乱，而产后持续光照可补偿 Cryptochrome1 和 2 双缺失对生物钟的影响。
PLoS One. 2013 Nov 20;8(11):e80615. doi: 10.1371/journal.pone.0080615. eCollection 2013.
4
Circadian PER2::LUC rhythms in the olfactory bulb of freely moving mice depend on the suprachiasmatic nucleus but not on behaviour rhythms.自由活动小鼠嗅球中的昼夜节律PER2::LUC节律依赖于视交叉上核，而非行为节律。
Eur J Neurosci. 2015 Dec;42(12):3128-37. doi: 10.1111/ejn.13111.
5
Effects of vasoactive intestinal peptide genotype on circadian gene expression in the suprachiasmatic nucleus and peripheral organs.血管活性肠肽基因型对下丘脑视交叉上核和外周器官昼夜节律基因表达的影响。
J Biol Rhythms. 2011 Jun;26(3):200-9. doi: 10.1177/0748730411401740.
6
Restoring the Molecular Clockwork within the Suprachiasmatic Hypothalamus of an Otherwise Clockless Mouse Enables Circadian Phasing and Stabilization of Sleep-Wake Cycles and Reverses Memory Deficits.在一只原本没有生物钟的小鼠的视交叉上核内恢复分子生物钟机制，能够实现昼夜节律的相位调整和睡眠-觉醒周期的稳定，并逆转记忆缺陷。
J Neurosci. 2021 Oct 13;41(41):8562-8576. doi: 10.1523/JNEUROSCI.3141-20.2021. Epub 2021 Aug 26.
7
Behavioural food anticipation in clock genes deficient mice: confirming old phenotypes, describing new phenotypes.生物钟基因缺失小鼠的行为性食物预期：确认旧表型，描述新表型。
Genes Brain Behav. 2010 Jul;9(5):467-77. doi: 10.1111/j.1601-183X.2010.00576.x. Epub 2010 Feb 17.
8
IA Channels Encoded by Kv1.4 and Kv4.2 Regulate Circadian Period of PER2 Expression in the Suprachiasmatic Nucleus.由Kv1.4和Kv4.2编码的IA通道调节视交叉上核中PER2表达的昼夜节律周期。
J Biol Rhythms. 2015 Oct;30(5):396-407. doi: 10.1177/0748730415593377. Epub 2015 Jul 6.
9
Differential roles of AVP and VIP signaling in the postnatal changes of neural networks for coherent circadian rhythms in the SCN.AVP 和 VIP 信号在 SCN 中昼夜节律相干神经网络的出生后变化中的差异作用。
Sci Adv. 2016 Sep 9;2(9):e1600960. doi: 10.1126/sciadv.1600960. eCollection 2016 Sep.
10
Restoration of circadian rhythmicity in circadian clock-deficient mice in constant light.在持续光照条件下恢复生物钟缺陷小鼠的昼夜节律性。
J Biol Rhythms. 2006 Jun;21(3):169-76. doi: 10.1177/0748730406288040.
引用本文的文献
1
Suprachiasmatic nucleus-wide estimation of oscillatory temporal dynamics.视交叉上核范围内振荡时间动态的估计。
PLoS Comput Biol. 2025 Mar 6;21(3):e1012855. doi: 10.1371/journal.pcbi.1012855. eCollection 2025 Mar.
2
Fiber photometry in neuroscience research: principles, applications, and future directions.神经科学研究中的光纤光度测定法：原理、应用及未来方向。
Pharmacol Rep. 2024 Dec;76(6):1242-1255. doi: 10.1007/s43440-024-00646-w. Epub 2024 Sep 5.
3
Light regulation of rhodopsin distribution during outer segment renewal in murine rod photoreceptors.光调控鼠视杆细胞外节更新过程中视紫红质的分布
Curr Biol. 2024 Apr 8;34(7):1492-1505.e6. doi: 10.1016/j.cub.2024.02.070. Epub 2024 Mar 19.
4
The Suprachiasmatic Nucleus at 50: Looking Back, Then Looking Forward.视交叉上核 50 年：回顾过去，展望未来。
J Biol Rhythms. 2024 Apr;39(2):135-165. doi: 10.1177/07487304231225706. Epub 2024 Feb 16.
5
In vivo recording of suprachiasmatic nucleus dynamics reveals a dominant role of arginine vasopressin neurons in circadian pacesetting.在体记录视交叉上核的动力学变化揭示了精氨酸加压素神经元在昼夜节律起搏中的主导作用。
PLoS Biol. 2023 Aug 29;21(8):e3002281. doi: 10.1371/journal.pbio.3002281. eCollection 2023 Aug.
6
A Journey in the Brain's Clock: In Vivo Veritas?大脑生物钟之旅：体内的真相？
Biology (Basel). 2023 Aug 15;12(8):1136. doi: 10.3390/biology12081136.
7
Ultrastructure of Synaptic Connectivity within Subregions of the Suprachiasmatic Nucleus Revealed by a Genetically Encoded Tag and Serial Blockface Electron Microscopy.利用遗传编码标签和连续块面电子显微镜技术揭示视交叉上核亚区突触连接的超微结构。
eNeuro. 2023 Aug 23;10(8). doi: 10.1523/ENEURO.0227-23.2023. Print 2023 Aug.
8
The circadian clock is disrupted in pancreatic cancer.昼夜节律钟在胰腺癌中被打乱。
PLoS Genet. 2023 Jun 1;19(6):e1010770. doi: 10.1371/journal.pgen.1010770. eCollection 2023 Jun.
9
Retinorecipient areas in the common marmoset (): An image-forming and non-image forming circuitry.普通狨猴的视网膜接受区（）：一个形成图像和非形成图像的回路。
Front Neural Circuits. 2023 Feb 2;17:1088686. doi: 10.3389/fncir.2023.1088686. eCollection 2023.
10
Nested calcium dynamics support daily cell unity and diversity in the suprachiasmatic nuclei of free-behaving mice.嵌套式钙动力学支持自由活动小鼠视交叉上核中细胞的日常统一性和多样性。
PNAS Nexus. 2022 Jul 11;1(3):pgac112. doi: 10.1093/pnasnexus/pgac112. eCollection 2022 Jul.
本文引用的文献
1
Calcium Circadian Rhythmicity in the Suprachiasmatic Nucleus: Cell Autonomy and Network Modulation.视交叉上核中的钙昼夜节律：细胞自主性和网络调节。
eNeuro. 2017 Aug 18;4(4). doi: 10.1523/ENEURO.0160-17.2017. eCollection 2017 Jul-Aug.
2
Dissociation of and circadian rhythms in the suprachiasmatic nucleus in parallel with behavioral outputs.视交叉上核中[具体物质]与昼夜节律的解离与行为输出平行。 （注：原文中“and”前面应该有具体物质未给出）
Proc Natl Acad Sci U S A. 2017 May 2;114(18):E3699-E3708. doi: 10.1073/pnas.1613374114. Epub 2017 Apr 17.
3
Synchronous circadian voltage rhythms with asynchronous calcium rhythms in the suprachiasmatic nucleus.视交叉上核中同步的时钟电压节律与异步的钙节律。
Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):E2476-E2485. doi: 10.1073/pnas.1616815114. Epub 2017 Mar 7.
4
Transcriptional architecture of the mammalian circadian clock.哺乳动物昼夜节律钟的转录结构
Nat Rev Genet. 2017 Mar;18(3):164-179. doi: 10.1038/nrg.2016.150. Epub 2016 Dec 19.
5
Visualizing and Quantifying Intracellular Behavior and Abundance of the Core Circadian Clock Protein PERIOD2.可视化和量化核心生物钟蛋白周期蛋白2（PERIOD2）的细胞内行为及丰度
Curr Biol. 2016 Jul 25;26(14):1880-6. doi: 10.1016/j.cub.2016.05.018. Epub 2016 Jun 30.
6
In vivo imaging of clock gene expression in multiple tissues of freely moving mice.在自由活动小鼠的多个组织中对生物钟基因表达进行体内成像。
Nat Commun. 2016 Jun 10;7:11705. doi: 10.1038/ncomms11705.
7
Circadian and ultradian rhythms of clock gene expression in the suprachiasmatic nucleus of freely moving mice.自由活动小鼠视交叉上核中时钟基因表达的昼夜节律和超日节律。
Sci Rep. 2015 Jul 21;5:12310. doi: 10.1038/srep12310.
8
Light evokes rapid circadian network oscillator desynchrony followed by gradual phase retuning of synchrony.光会引发昼夜节律网络振荡器的快速去同步，随后是同步性的逐渐相位重新调整。
Curr Biol. 2015 Mar 30;25(7):858-67. doi: 10.1016/j.cub.2015.01.056. Epub 2015 Mar 5.
9
Mice genetically deficient in vasopressin V1a and V1b receptors are resistant to jet lag.血管加压素 V1a 和 V1b 受体基因缺失的小鼠对时差有抵抗力。
Science. 2013 Oct 4;342(6154):85-90. doi: 10.1126/science.1238599.
10
Real-time recording of circadian liver gene expression in freely moving mice reveals the phase-setting behavior of hepatocyte clocks.实时记录自由活动小鼠肝脏的昼夜基因表达，揭示了肝细胞时钟的相位设定行为。
Genes Dev. 2013 Jul 1;27(13):1526-36. doi: 10.1101/gad.221374.113.
Zotero 插件