Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.
Cell Prolif. 2022 Oct;55(10):e13287. doi: 10.1111/cpr.13287. Epub 2022 Jul 17.
The emergence of single-cell RNA sequencing enables simultaneous sequencing of thousands of cells, making the analysis of cell population heterogeneity more efficient. In recent years, single-cell RNA sequencing has been used in the investigation of heterogeneous cell populations, cellular developmental trajectories, stochastic gene transcriptional kinetics, and gene regulatory networks, providing strong support in life science research. However, the application of single-cell RNA sequencing in the field of oral science has not been reviewed comprehensively yet. Therefore, this paper reviews the development and application of single-cell RNA sequencing in oral science, including fields of tissue development, teeth and jaws diseases, maxillofacial tumors, infections, etc., providing reference and prospects for using single-cell RNA sequencing in studying the oral diseases, tissue development, and regeneration.
单细胞 RNA 测序的出现使得同时对数千个细胞进行测序成为可能，从而使细胞群体异质性的分析更加高效。近年来，单细胞 RNA 测序已被应用于异质细胞群体、细胞发育轨迹、随机基因转录动力学和基因调控网络的研究中，为生命科学研究提供了有力支持。然而，单细胞 RNA 测序在口腔科学领域的应用尚未得到全面综述。因此，本文综述了单细胞 RNA 测序在口腔科学中的发展和应用，包括组织发育、牙齿和颌骨疾病、颌面肿瘤、感染等领域，为使用单细胞 RNA 测序研究口腔疾病、组织发育和再生提供了参考和前景。
