采用气相色谱 - 电子捕获检测法简单灵敏地测定生物样品中的甲基乙二醛。
Simple and sensitive determination of methylglyoxal in biological samples by gas chromatography with electron-capture detection.
作者信息
Ohmori S, Kawase M, Mori M, Hirota T
出版信息
J Chromatogr. 1987 Apr 10;415(2):221-9. doi: 10.1016/s0378-4347(00)83214-4.
Methylglyoxal was allowed to react with 4,5-dichloro-1,2-phenylenediamine, and the 6,7-dichloro-2-methylquinoxaline formed was determined by gas chromatography with electron-capture detection. The standard curve of the quinoxaline was linear up to 160 pmol/ml. The recoveries of methylglyoxal from coffee and rat liver homogenate were 84.1 and 77.6%, respectively. This procedure was very selective and so sensitive that as little as 9 fmol of the quinoxaline could be measured in biological and food samples.
使甲基乙二醛与4,5-二氯-1,2-苯二胺反应，通过带有电子捕获检测的气相色谱法测定生成的6,7-二氯-2-甲基喹喔啉。喹喔啉的标准曲线在高达160 pmol/ml时呈线性。甲基乙二醛在咖啡和大鼠肝脏匀浆中的回收率分别为84.1%和77.6%。该方法具有很高的选择性且非常灵敏，以至于在生物和食品样品中能检测到低至9 fmol的喹喔啉。
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