Larsson L, Odham G, Westerdahl G, Olsson B
J Clin Microbiol. 1987 May;25(5):893-6. doi: 10.1128/jcm.25.5.893-896.1987.
Coded sputum specimens from patients with tuberculous (8 specimens) and nontuberculous (15 specimens) pneumonia were analyzed for tuberculostearic acid by selected-ion monitoring of the pentafluorobenzyl ester derivative by using negative-ion mass spectrometry. The analytical results agreed with those from culturing of the specimens, except in three cases in which the chromatograms were difficult to interpret owing to insufficient amounts of sputa available. Negative-ion mass spectrometry provided superior sensitivity and selectivity for detection of tuberculostearic acid compared with the previously used gas chromatographic-mass spectrometric methods. The technique reported here should have considerable potential for the rapid diagnosis of pulmonary tuberculosis by direct analysis of sputum.
采用负离子质谱法通过对五氟苄酯衍生物进行选择离子监测，分析了患有结核性肺炎（8份样本）和非结核性肺炎（15份样本）患者的编码痰液样本中的结核硬脂酸。分析结果与样本培养结果一致，但有3例因可用痰液量不足导致色谱图难以解读。与之前使用的气相色谱 - 质谱法相比，负离子质谱法在检测结核硬脂酸方面具有更高的灵敏度和选择性。本文报道的技术通过直接分析痰液对肺结核的快速诊断应具有相当大的潜力。
