采用电子捕获检测的二维气相色谱法用于灵敏测定特定分枝杆菌脂质成分。
Two-dimensional gas chromatography with electron capture detection for the sensitive determination of specific mycobacterial lipid constituents.
作者信息
Larsson L, Jimenez J, Sonesson A, Portaels F
机构信息
Department of Medical Microbiology, Lund University Hospital, Sweden.
出版信息
J Clin Microbiol. 1989 Oct;27(10):2230-3. doi: 10.1128/jcm.27.10.2230-2233.1989.
Abstract
A method was developed for determining two characteristic mycobacterial lipid constituents, tuberculostearic acid (as its pentafluorobenzyl ester) and 2-eicosanol (as its pentafluorobenzoyl ester), by using gas chromatography with electron capture detection. A microprocessor-controlled column-switching system (two-dimensional gas chromatography) facilitated sample preparation and increased specificity. The usefulness of the technique was illustrated by its ability to reveal picogram amounts of tuberculostearate in a suspension of Mycobacterium leprae isolated from a naturally infected armadillo. Two-dimensional gas chromatography with electron capture detection may in some instances provide a convenient alternative to gas chromatography-mass spectrometry for use in demonstrating the presence of mycobacteria in a complex environment.
摘要
开发了一种通过使用带电子捕获检测的气相色谱法来测定两种特征性分枝杆菌脂质成分的方法，即结核硬脂酸（以其五氟苄酯形式）和2-二十烷醇（以其五氟苯甲酰酯形式）。微处理器控制的柱切换系统（二维气相色谱）便于样品制备并提高了特异性。该技术的实用性通过其能够在从自然感染的犰狳分离出的麻风分枝杆菌悬浮液中检测到皮克量的结核硬脂酸盐得到了说明。在某些情况下，带电子捕获检测的二维气相色谱法可能为气相色谱-质谱联用提供一种方便的替代方法，用于在复杂环境中证明分枝杆菌的存在。
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本文引用的文献
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Use of gas chromatography to differentiate Mycobacterium leprae from cultivable armadillo-derived mycobacteria, M. avium/intracellulare, and M. lepraemurium by analysis of secondary alcohols.通过分析仲醇，利用气相色谱法区分麻风分枝杆菌与可培养的犰狳源分枝杆菌、鸟分枝杆菌/胞内分枝杆菌以及鼠麻风分枝杆菌。
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J Chromatogr. 1987 Jun 5;417(1):11-25. doi: 10.1016/0378-4347(87)80087-7.
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