Li Tao, Liu Zhenyu, Wang Yitong, Zuo Dongshi, Wang Shenyuan, Ju Haitao, Wang Shichao, Xing Yanping, Ling Yu, Liu Chunxia, Zhang Yanru, Zhou Huanmin, Yin Jun, Cao Junwei, Gao Jing
Inner Mongolia Key Laboratory of Bio-manufacture, College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, China.
Inner Mongolia Autonomous Region Key Laboratory of Big Data Research and Application of Agriculture and Animal Husbandry, College of Computer and Information Engineering, Inner Mongolia Agricultural University, Hohhot, China.
Front Neurosci. 2022 Jul 1;16:877802. doi: 10.3389/fnins.2022.877802. eCollection 2022.
Understanding the complete map of melatonin synthesis, the information transfer network among circadian genes in pineal gland, promises to resolve outstanding issues in endocrine systems and improve the clinical diagnosis and treatment level of insomnia, immune disease and hysterical depression. Currently, some landmark studies have revealed some genes that regulate circadian rhythm associated with melatonin synthesis. However, these studies don't give a complete map of melatonin synthesis, as transfer information among circadian genes in pineal gland is lost. New biotechnology, integrates dynamic sequential omics and multiplexed imaging method, has been used to visualize the complete process of melatonin synthesis. It is found that there are two extremely significant information transfer processes involved in melatonin synthesis. In the first stage, as the light intensity decreased, melatonin synthesis mechanism has started, which is embodied in circadian genes, , and become active. In the second stage, circadian genes , and are regulated simultaneously by four genes, , and and contribute genetic information to . The expeditious growth in this technique offer reference for an overall understanding of gene-to-gene regulatory relationship among circadian genes in pineal gland. In the study, dynamic sequential omics and the analysis process well provide the current state and future perspectives to better diagnose and cure diseases associated with melatonin synthesis disorder.
了解褪黑素合成的完整图谱，即松果体中昼夜节律基因之间的信息传递网络，有望解决内分泌系统中的突出问题，并提高失眠、免疫疾病和癔症性抑郁症的临床诊断和治疗水平。目前，一些具有里程碑意义的研究已经揭示了一些与褪黑素合成相关的调节昼夜节律的基因。然而，这些研究并没有给出褪黑素合成的完整图谱，因为松果体中昼夜节律基因之间的信息传递丢失了。整合了动态序列组学和多重成像方法的新生物技术已被用于可视化褪黑素合成的完整过程。研究发现，褪黑素合成涉及两个极其重要的信息传递过程。在第一阶段，随着光强度降低，褪黑素合成机制启动，这体现在昼夜节律基因、和变得活跃。在第二阶段，昼夜节律基因、和同时受到四个基因、和的调控，并为贡献遗传信息。这项技术的迅速发展为全面了解松果体中昼夜节律基因之间的基因对基因调控关系提供了参考。在该研究中，动态序列组学及其分析过程很好地提供了当前状态和未来前景，以便更好地诊断和治疗与褪黑素合成紊乱相关的疾病。
