El Husseiny Iman M, El Kholy Samar, Mohamed Amira Z, Meshrif Wesam S, Elbrense Hanaa
Department of Zoology, Faculty of Science, Tanta University, 31527 Tanta, Egypt.
Faculty of Medicine, Tanta University, 31527 Tanta, Egypt.
Saudi J Biol Sci. 2022 May;29(5):3739-3748. doi: 10.1016/j.sjbs.2022.03.006. Epub 2022 Mar 11.
While holding on youth may be a universal wish, aging is a natural process associated with physical and physiological impairment in living organisms. provides useful insights into aging-related events. Hence, this study was conducted to investigate the age-related changes in muscle function and architecture in relation to the biogenic amine titers. To achieve this aim, visceral and skeletal muscles performance was tested in newly-eclosed, sexually mature and old adult flies using climbing and gut motility assays. In addition, age-related ultrastructural alterations of muscular tissue were observed using transmission electron microscopy (TEM). The titer of selected biogenic amines was measured using high-performance liquid chromatography (HPLC). The results demonstrated that old flies were dramatically slower in upward movement than either newly-eclosed or sexually mature flies. Similarly, gut contraction rate was significantly lower in old flies than the sexually mature, although it was markedly higher than that in the newly-eclosed flies. In TEM examination, there were several ultrastructural changes in the midgut epithelium, legs and thorax muscles of old flies. Regarding biogenic amine titers, the old flies had significantly lower concentrations of octopamine, dopamine and serotonin than the sexually mature. We concluded that aging has adverse effects on muscular system function and ultrastructure, synchronized with biogenic amine titers changes. Our results highlighted the need for more researches on therapeutics that may balance the levels of age-related alterations in biogenic amines.
尽管留住青春可能是普遍的愿望，但衰老是一个与生物体身体和生理损伤相关的自然过程。它为与衰老相关的事件提供了有用的见解。因此，本研究旨在调查与生物胺滴度相关的肌肉功能和结构的年龄相关变化。为实现这一目标，使用攀爬和肠道运动测定法对新羽化、性成熟和老年成年果蝇的内脏和骨骼肌性能进行了测试。此外，使用透射电子显微镜（TEM）观察了肌肉组织与年龄相关的超微结构变化。使用高效液相色谱（HPLC）测量选定生物胺的滴度。结果表明，老年果蝇向上移动的速度比新羽化或性成熟的果蝇慢得多。同样，老年果蝇的肠道收缩率明显低于性成熟果蝇，尽管明显高于新羽化果蝇。在TEM检查中，老年果蝇的中肠上皮、腿部和胸部肌肉有几种超微结构变化。关于生物胺滴度，老年果蝇的章鱼胺、多巴胺和血清素浓度明显低于性成熟果蝇。我们得出结论，衰老对肌肉系统功能和超微结构有不利影响，与生物胺滴度变化同步发生。我们的结果强调需要对可能平衡与年龄相关的生物胺变化水平的治疗方法进行更多研究。
