Hardie Shannon L, Hirsh Jay
Department of Biology and Neuroscience Graduate Program, University of Virginia, 262 Gilmer Hall, Charlottesville, VA 22903, United States.
J Neurosci Methods. 2006 Jun 15;153(2):243-9. doi: 10.1016/j.jneumeth.2005.11.001. Epub 2005 Dec 6.
Biogenic amines are critically important neuromodulators in both vertebrates and invertebrates. Quantification of these amines can be difficult, particularly in neural extracts of Drosophila melanogaster that contain interfering electroactive compounds. We have developed a method for the reliable separation and quantification of the biogenic amines dopamine, serotonin, tyramine, and octopamine in Drosophila brain extracts using high performance liquid chromatography with electrochemical detection. Our method obviates the need for complex preparatory procedures or instrumentation, and can reproducibly detect picogram quantities of these amines. By optimizing the composition of the mobile phase and the electrode potential, and by examining common complications in the analysis of biological samples, we have developed a reliable technique for monitoring levels of biogenic amines in the Drosophila brain.
生物胺在脊椎动物和无脊椎动物中都是至关重要的神经调节剂。对这些胺类进行定量分析可能具有挑战性，尤其是在含有干扰性电活性化合物的黑腹果蝇神经提取物中。我们开发了一种方法，利用高效液相色谱与电化学检测联用技术，可靠地分离和定量黑腹果蝇脑提取物中的生物胺多巴胺、血清素、酪胺和章鱼胺。我们的方法无需复杂的制备程序或仪器，并且能够重复检测皮克级数量的这些胺类。通过优化流动相组成和电极电位，并研究生物样品分析中的常见问题，我们开发了一种可靠的技术来监测果蝇脑中生物胺的水平。
