Siregar Adrian S, Nyiramana Marie Merci, Kim Eun-Jin, Shin Eui-Jung, Woo Min Seok, Kim Jin-Mok, Park Si-Hyang, Hahm Jong Ryeal, Choi Yeung Joon, Kang Dawon
Department of Physiology and Institute of Health Sciences College of Medicine Gyeongsang National University Jinju South Korea.
Department of Convergence Medical Science Gyeongsang National University Jinju South Korea.
Food Sci Nutr. 2022 Mar 29;10(7):2390-2399. doi: 10.1002/fsn3.2847. eCollection 2022 Jul.
Our previous study showed that oyster hydrolysate (OH) protected against the liver damage caused by a single instance of ethanol (EtOH) binge drinking. Oyster broth concentrate (OBC) was discovered in the process of searching for a different substance derived from oysters () with economic value. OBC is a by-product of boiling oysters at 95°C for 3 min. In this study, we investigated the effects of OBC and its major component taurine on blood and liver tissues obtained from a single-EtOH-binge-drinking mouse model. The preadministration of OBC enhanced EtOH metabolism by increasing the activities of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), and catalase. In addition, the preadministration of OBC reduced cytochrome P450 2E1 (CYP2E1) activity, reactive oxygen species (ROS) generation, Ca concentrations, apoptotic signals, and inflammatory mediators in liver tissues. The reduction of apoptotic and inflammatory signals by OBC resulted from the downregulation of endoplasmic reticulum (ER) stress molecules and NF-κB activity. Taurine administration showed similar effects to OBC. These results show that OBC protected against acute EtOH-induced liver damage through the action of taurine. Our findings suggest that OBC could be an economically valuable substance and a functional food with hepatoprotective effects.
我们之前的研究表明，牡蛎水解物（OH）可预防单次乙醇（EtOH）暴饮所致的肝损伤。在寻找具有经济价值的不同牡蛎衍生物质的过程中发现了牡蛎浓缩汤（OBC）。OBC是将牡蛎在95°C煮3分钟后的副产品。在本研究中，我们研究了OBC及其主要成分牛磺酸对单次乙醇暴饮小鼠模型的血液和肝脏组织的影响。预先给予OBC可通过提高乙醇脱氢酶（ADH）、乙醛脱氢酶（ALDH）和过氧化氢酶的活性来增强乙醇代谢。此外，预先给予OBC可降低肝脏组织中细胞色素P450 2E1（CYP2E1）活性、活性氧（ROS）生成、钙浓度、凋亡信号和炎症介质。OBC对凋亡和炎症信号的减少是由于内质网（ER）应激分子和NF-κB活性的下调所致。给予牛磺酸显示出与OBC相似的效果。这些结果表明，OBC通过牛磺酸的作用预防急性乙醇诱导的肝损伤。我们的研究结果表明，OBC可能是一种具有经济价值的物质和具有肝保护作用的功能性食品。
