Ryu Ji Hyeon, Woo Min Seok, Cao Dang Long, Kim Eun-Jin, Jeong Yi Yeong, Koh Eun-Ha, Cho Kye Man, Kang Sang Soo, Kang Dawon
Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan 50612, Korea.
Department of Physiology, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52727, Korea.
Antioxidants (Basel). 2022 Oct 18;11(10):2052. doi: 10.3390/antiox11102052.
The association between asthma and oxidative stress remains controversial. Oxidative stress-induced ferroptosis has not been extensively studied in asthma models. This study was performed to investigate the anti-asthmatic and anti-ferroptotic effects of fermented and aged ginseng sprouts (FAGS) with enhanced antioxidant activity and its main component, compound K (CK), in a mouse model of ovalbumin (OVA)-induced allergic asthma. The experimental asthma model was sensitized and challenged with OVA. During the challenge period, two different concentrations of FAGS and CK were administered via oral gavage. Asthmatic parameters were analyzed in bronchoalveolar lavage fluid (BALF), blood, and lung tissue. CK, among the ginsenosides analyzed, was highly increased in FAGS compared with GS. Asthma parameters, such as Th2 cytokine and IgE production, mast cell activation, goblet cell hyperplasia, hyperresponsiveness, and inflammation, were dramatically increased in the OVA group. Oxidation and ferroptosis markers were increased in the OVA group. The asthma parameters and ferroptosis markers were markedly decreased in the OVA + FAGS and OVA + CK groups. These results showed that FAGS and CK alleviated asthma parameters in an allergic asthma mouse model by inhibiting inflammation and ferroptosis. Our findings suggest that FAGS and CK could be used as potential treatments for allergic asthma.
哮喘与氧化应激之间的关联仍存在争议。氧化应激诱导的铁死亡在哮喘模型中尚未得到广泛研究。本研究旨在探讨具有增强抗氧化活性的发酵和陈化人参芽（FAGS）及其主要成分化合物K（CK）在卵清蛋白（OVA）诱导的过敏性哮喘小鼠模型中的抗哮喘和抗铁死亡作用。实验性哮喘模型用OVA致敏并激发。在激发期间，通过灌胃给予两种不同浓度的FAGS和CK。对支气管肺泡灌洗液（BALF）、血液和肺组织中的哮喘参数进行分析。在所分析的人参皂苷中，与GS相比，FAGS中的CK含量显著增加。OVA组中Th2细胞因子和IgE产生、肥大细胞活化、杯状细胞增生、高反应性和炎症等哮喘参数显著增加。OVA组中氧化和铁死亡标志物增加。OVA + FAGS组和OVA + CK组的哮喘参数和铁死亡标志物显著降低。这些结果表明，FAGS和CK通过抑制炎症和铁死亡减轻了过敏性哮喘小鼠模型中的哮喘参数。我们的研究结果表明，FAGS和CK可用作过敏性哮喘的潜在治疗方法。
