Tian Yuan, Yang Tiezhu, Yang Shijie, Wang Leping, Zhao Liangjie, Sun Wenhao
School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, China.
Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang, China.
Mitochondrial DNA B Resour. 2022 Jul 12;7(7):1288-1290. doi: 10.1080/23802359.2022.2097487. eCollection 2022.
The complete mitogenome sequence of (Kreyenberg & Pappenheim, 1908) from Wabu Lake in Huai River Basin was annotated and characterized in this study. This mitochondrial genome is a circular DNA molecule of 16.896 bp in size with 57.52% AT content, including 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an AT-rich region (control region) as other bony fishes. There are a total of 10 overlap locations and 15 intergenic spacer regions throughout the mitogenome of . All PCGs employed a standard ATG as a start codon, except cytochrome c oxidase 1 (cox1) with GTG. In addition, TAA or TAG was identified as the typical stop codon. A phylogenetic tree reconstructed with the maximum likelihood method depicted a clone relationship with eight species of genus and our previous study based on the amino acid sequences of 13 mitochondrial PCGs. The complete mitochondrial genome is a valuable resource in classifying and conserving .
本研究对采自淮河流域瓦埠湖的（克雷恩贝格和帕彭海姆，1908年）的完整线粒体基因组序列进行了注释和特征分析。该线粒体基因组是一个大小为16,896 bp的环状DNA分子，AT含量为57.52%，与其他硬骨鱼类一样，包括13个蛋白质编码基因（PCGs）、两个核糖体RNA基因（rRNAs）、22个转移RNA基因（tRNAs）和一个富含AT的区域（控制区）。在该物种的线粒体基因组中共有10个重叠位置和15个基因间隔区。除细胞色素c氧化酶1（cox1）以GTG作为起始密码子外，所有PCGs均以标准的ATG作为起始密码子。此外，TAA或TAG被确定为典型的终止密码子。用最大似然法重建的系统发育树显示，基于13个线粒体PCGs的氨基酸序列，该物种与该属的8个物种以及我们之前的研究呈现出克隆关系。完整的线粒体基因组是该物种分类和保护的宝贵资源。
