Ruat Julia, Genewsky Andreas J, Heinz Daniel E, Kaltwasser Sebastian F, Canteras Newton S, Czisch Michael, Chen Alon, Wotjak Carsten T
Department Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, 80804 Munich, Germany.
Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, 80804 Munich, Germany.
iScience. 2022 Jun 22;25(7):104657. doi: 10.1016/j.isci.2022.104657. eCollection 2022 Jul 15.
Although mice mostly communicate in the ultrasonic range, they also emit audible calls. We demonstrate that mice selectively bred for high anxiety-related behavior (HAB) have a high disposition for emitting sonic calls when caught by the tail. The vocalization was unrelated to pain but sensitive to anxiolytics. As revealed by manganese-enhanced MRI, HAB mice displayed an increased tonic activity of the periaqueductal gray (PAG). Selective inhibition of the dorsolateral PAG not only reduced anxiety-like behavior but also completely abolished sonic vocalization. Calls were emitted at a fundamental frequency of 3.8 kHz, which falls into the hearing range of numerous predators. Indeed, playback of sonic vocalization attracted rats if associated with a stimulus mouse. If played back to HAB mice, sonic calls were repellent in the absence of a conspecific but attractive in their presence. Our data demonstrate that sonic vocalization attracts both predators and conspecifics depending on the context.
尽管小鼠大多在超声波范围内进行交流，但它们也会发出可听见的叫声。我们证明，经过选择性培育具有高焦虑相关行为（HAB）的小鼠在被抓住尾巴时发出声音叫声的倾向很高。这种发声与疼痛无关，但对抗焦虑药敏感。正如锰增强磁共振成像所显示的，HAB小鼠中脑导水管周围灰质（PAG）的紧张性活动增加。选择性抑制背外侧PAG不仅减少了焦虑样行为，还完全消除了声音发声。叫声以3.8千赫的基频发出，这落在许多捕食者的听力范围内。事实上，如果与一只刺激小鼠相关联，声音发声的回放会吸引大鼠。如果回放给HAB小鼠，声音叫声在没有同种个体时具有排斥性，但在有同种个体时具有吸引力。我们的数据表明，声音发声根据情境既吸引捕食者也吸引同种个体。
