Maleki Bahman, Sadraei Javid, Dalimi Asl Abdolhossein, Pirestani Majid
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2022 Spring;15(2):164-171.
The current study investigated the prevalence and genotypes of sp. in individuals who referred to medical laboratories in Kermanshah, Iran.
sp is a common intestinal protozoan found in humans and a wide range of animals, and it is involved in the development of gastrointestinal disorders.
A total of 950 stool samples were examined using the standard formalin-ether concentration technique. All specimens were cultured in Robinson xenic medium. Subsequently, DNA extraction and PCR amplification of subtype specific sequence-tagged site (STS) were conducted.
Microscopic examination showed that 86 out of 950 samples (9.05%) were infected with sp. Subsequently, 33 of 86 positive samples were cultured and molecularly confirmed by conventional PCR, indicating six subtypes (ST1-ST6). Of note, ST3 (45.0%) was the predominant subtype, followed by ST1 (15.15%) and ST5 (12%).
Based on the current findings, ST3 was the most frequent subtype among all positive samples. Having a better understanding of sp. subtype distribution and risk factors would lead to improved preventive measures.
本研究调查了转诊至伊朗克尔曼沙赫医学实验室的个体中 虫种的流行情况和基因型。
虫是一种常见的肠道原生动物，存在于人类和多种动物体内，与胃肠道疾病的发生有关。
采用标准的福尔马林 - 乙醚浓缩技术对950份粪便样本进行检查。所有标本均在罗宾逊异种培养基中培养。随后进行亚型特异性序列标签位点（STS）的DNA提取和PCR扩增。
显微镜检查显示，950份样本中有86份（9.05%）感染了 虫。随后，对86份阳性样本中的33份进行培养，并通过常规PCR进行分子确认，确定了六个亚型（ST1 - ST6）。值得注意的是，ST3（45.0%）是主要亚型，其次是ST1（15.15%）和ST5（12%）。
根据目前的研究结果，ST3是所有阳性样本中最常见的亚型。更好地了解 虫的亚型分布和危险因素将有助于改进预防措施。
