Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Plant Cell Environ. 2019 Apr;42(4):1340-1351. doi: 10.1111/pce.13470. Epub 2018 Nov 29.
Tartary buckwheat (Fagopyrum tataricum) not only provides a supplement to primary grain crops in China but also has high medicinal value, by virtue of its rich content of flavonoids possessing antioxidant, anti-inflammatory, and anticancer properties. Light is an important environmental factor that can regulate the synthesis of plant secondary metabolites. In this study, we treated tartary buckwheat seedlings with different wavelengths of light and found that red and blue light could increase the content of flavonoids and the expression of genes involved in flavonoid synthesis pathways. Through coexpression analysis, we identified a new MYB transcription factor (FtMYB116) that can be induced by red and blue light. Yeast one-hybrid assays and an electrophoretic mobility shift assay showed that FtMYB116 binds directly to the promoter region of flavonoid-3'-hydroxylase (F3'H), and a transient luciferase activity assay indicated that FtMYB116 can induce F3'H expression. After transforming FtMYB116 into the hairy roots of tartary buckwheat, we observed significant increases in the content of rutin and quercetin. Collectively, our results indicate that red and blue light promote an increase in flavonoid content in tartary buckwheat seedlings; we also identified a new MYB transcription factor, FtMYB116, that promotes the accumulation of rutin via direct activation of F3'H expression.
鞑靼荞麦不仅为中国的主要粮食作物提供了补充，而且还具有很高的药用价值，因为它含有丰富的类黄酮，具有抗氧化、抗炎和抗癌特性。光作为一种重要的环境因素，可以调节植物次生代谢物的合成。在这项研究中，我们用不同波长的光处理鞑靼荞麦幼苗，发现红光和蓝光可以增加类黄酮的含量和参与类黄酮合成途径的基因的表达。通过共表达分析，我们鉴定了一种新的 MYB 转录因子（FtMYB116），它可以被红光和蓝光诱导。酵母单杂交试验和电泳迁移率变动分析表明，FtMYB116 直接结合到类黄酮-3'-羟化酶（F3'H）的启动子区域，瞬时萤光素酶活性试验表明，FtMYB116 可以诱导 F3'H 的表达。在将 FtMYB116 转化到鞑靼荞麦的毛状根中后，我们观察到芦丁和槲皮素的含量显著增加。总之，我们的结果表明，红光和蓝光促进了鞑靼荞麦幼苗中类黄酮含量的增加；我们还鉴定了一种新的 MYB 转录因子 FtMYB116，它可以通过直接激活 F3'H 的表达来促进芦丁的积累。
