LAQV-REQUIMTE, Department of Chemical Sciences, Faculty of Farmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
LAQV-REQUIMTE, Department of Chemical Sciences, Faculty of Farmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; FCS/UFP, Faculty of Health Sciences, University Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal.
Food Chem. 2018 Sep 1;259:113-121. doi: 10.1016/j.foodchem.2018.03.113. Epub 2018 Mar 27.
The present study reports for the first time the identification and quantification of phenolic compounds, the antioxidant and antimicrobial activities as well as the in vitro radical scavenging activity and intestinal cell effects of A. arguta leaves extracts. Extractions were carried out under water, water:ethanol (50:50) and ethanol. The highest antioxidant activity were obtained in alcoholic extract (IC = 53.95 ± 3.09 μg/mL for DPPH; 6628.42 ± 382.49 µmol/mg dry weight basis for FRAP) while the phenolic profile confirmed by HPLC analysis revealed highest amounts of phenolic acids (hydroxycinnamic acid derivatives) and flavonoids (flavan-3-ol and flavonols derivatives). An excellent scavenging activity against reactive oxygen and nitrogen species were determined for all extracts as well as no adverse effects on Caco-2 and HT29-MTX cells in concentrations below 100 μg/mL and 1000 μg/mL, respectively. These results highlight the potentialities of hardy kiwi leaves valorization.
本研究首次报道了 A. arguta 叶提取物中酚类化合物的鉴定和定量、抗氧化和抗菌活性以及体外自由基清除活性和肠细胞效应。分别在水、水:乙醇（50:50）和乙醇中进行提取。在醇提取物中获得了最高的抗氧化活性（DPPH 的 IC = 53.95 ± 3.09 μg/mL；FRAP 的 6628.42 ± 382.49 μmol/mg 干重基础），而通过 HPLC 分析确认的酚类图谱显示出最高量的酚酸（羟基肉桂酸衍生物）和类黄酮（黄烷-3-醇和黄酮醇衍生物）。所有提取物均表现出对活性氧和氮物种的出色清除活性，并且在低于 100 μg/mL 和 1000 μg/mL 时，对 Caco-2 和 HT29-MTX 细胞均无不良影响。这些结果突出了耐寒猕猴桃叶利用的潜力。
