Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, China.
College of Agriculture, Yanbian University, Yanji, China.
Front Cell Infect Microbiol. 2022 Jun 30;12:938576. doi: 10.3389/fcimb.2022.938576. eCollection 2022.
To explore the specific virome, 6400 C were collected in Honghe autonomous prefecture, China. Abundant virus sequences were obtained from 28 viral families using metavirome sequencing. Herein, several viruses in virome were verified using the PCR technique, which covers Japanese encephalitis virus (JEV), Getah virus, and even Chikungunya virus (CHIKV). Seven JEV gene sequences were amplified successfully, of which JEV-China/CT2016E-1 shared the highest homology with the known JEV sequence isolated in Korea, 1946, with at least 96.1% nucleotide (nt) identity, which belonged to genotype III. Nine CHIKV gene sequences were amplified, which shared the highest with at least 93.0% nt identity with CHIKV from Thailand isolated in 2007, which was assigned to genotype Asian. Remarkably, CHIKV was isolated from in China for the first time. It was initially confirmed that the isolated virus CHIKV-China/CT2016-1 may increase infectivity after passaging in Vero cells from BHK-21 cells. Collectively, our study reveals the diversity, properties, and potential virus susceptibility dynamics of the virome and sheds new perspectives on the viral ecology in other important biological vectors.
为了探索特定的病毒组，在中国红河自治州采集了 6400 个样本。通过宏病毒组测序，从 28 个病毒科中获得了丰富的病毒序列。在此基础上，使用 PCR 技术验证了病毒组中的几种病毒，包括日本脑炎病毒（JEV）、基孔肯雅热病毒（CHIKV）等。成功扩增了 7 条 JEV 基因序列，其中 JEV-China/CT2016E-1 与韩国 1946 年分离的已知 JEV 序列的同源性最高，核苷酸（nt）同一性至少为 96.1%，属于基因型 III。扩增了 9 条 CHIKV 基因序列，与 2007 年泰国分离的 CHIKV 的 nt 同一性最高，至少为 93.0%，属于亚洲基因型。值得注意的是，首次从中国分离到 CHIKV。最初证实，从 BHK-21 细胞传代到 Vero 细胞后，分离的病毒 CHIKV-China/CT2016-1 可能增加了感染力。总的来说，我们的研究揭示了病毒组的多样性、特性和潜在的病毒易感性动态，并为其他重要生物载体中的病毒生态学提供了新的视角。
