Xiao Pengpeng, Hao Yujia, Yuan Yuge, Ma Wenzhou, Li Yiquan, Zhang He, Li Nan
Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, China.
Academician Workstation of Jilin Province, Changchun University of Chinese Medicine, Changchun, China.
Virulence. 2025 Dec;16(1):2444686. doi: 10.1080/21505594.2024.2444686. Epub 2024 Dec 23.
We studied the viromes of three dominant mosquito species in Wenzhou, a coastal city in Zhejiang Province, using metavirome sequencing, with 18 viral families identified. Viral sequences were verified by RT-PCR. The JEV E gene was most closely related to the 1988 Korean strain. DENV sequences were most closely related to the 1997 Australian strain. CHIKV-E1-1 was most closely related to the 1983 Senegal strain and belonged to West African genotype CHIKV. Remarkably, this is the first time that a West African genotype of CHIKV has been detected in Zhejiang Province. Mutations in the CHIKV-E1-1 protein A226V may increase infectivity in . Three non-conservative mutations of CHIKV-E1-1 (D45H, D70H and V290D) may have an impact on the function. In conclusion, our study reveals the diversity of mosquito-borne viruses and potential emerging outbreaks in the southeast coastal region of China, providing new perspectives for mining the ecological characterization of other important arboviruses.
我们利用宏病毒组测序技术研究了浙江省沿海城市温州三种主要蚊虫的病毒组，鉴定出18个病毒科。病毒序列通过逆转录聚合酶链反应（RT-PCR）进行验证。日本脑炎病毒（JEV）E基因与1988年韩国毒株关系最为密切。登革病毒（DENV）序列与1997年澳大利亚毒株关系最为密切。基孔肯雅病毒（CHIKV）E1-1与1983年塞内加尔毒株关系最为密切，属于西非基因型CHIKV。值得注意的是，这是首次在浙江省检测到西非基因型的CHIKV。CHIKV-E1-1蛋白中的A226V突变可能会增加其在……中的感染性。CHIKV-E1-1的三个非保守突变（D45H、D70H和V290D）可能会影响其功能。总之，我们的研究揭示了中国东南沿海地区蚊媒病毒的多样性和潜在的新发病例，为挖掘其他重要虫媒病毒的生态特征提供了新的视角。
