三带喙库蚊和致倦库蚊对鸭坦布苏病毒的媒介效能。
Vector competence of Culex tritaeniorhynchus and Culex quinquefasciatus (Diptera: Culicidae) for duck Tembusu virus transmission.
机构信息
Animal Vector-Borne Disease Research Unit, Veterinary Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Animal Vector-Borne Disease Research Unit, Veterinary Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
出版信息
Acta Trop. 2021 Feb;214:105785. doi: 10.1016/j.actatropica.2020.105785. Epub 2020 Dec 9.
Duck Tembusu virus (DTMUV), an emerging infectious disease in ducks, was detected in Culex (Cx.) tritaeniorhynchus mosquitoes collected from a duck farm; however, the exact role of mosquitoes in the ecology of DTMUV in Thailand remains unclear. Vector competence of Cx. tritaeniorhynchus and Cx. quinquefasciatus was examined for DTMUV. Cx. tritaeniorhynchus mosquitoes were allowed to feed on four levels (10, 10, 10, and 10 TCID/mL) of DTMUV, while Cx. quinquefasciatus were allowed to feed on two levels (10 and 10 TCID/mL) of DTMUV. Infection rates in Cx. tritaeniorhynchus were 1.6, 10.2, 35.8, and 59.3% after feeding on 10, 10, 10, and 10 TCID/mL of DTMUV, respectively, while dissemination and transmission were 20.3 and 16.9% after feeding on 10 TCID/mL of DTMUV. Infection rates in Cx. quinquefasciatus were 2.5 and 2.3% after feeding on 10 and 10 TCID/mL of DTMUV, respectively, with no virus dissemination and transmission found in all tested mosquitoes. Another study was conducted to examine the transovarial transmission of DTMUV in Cx. tritaeniorhynchus. Mosquitoes were allowed to feed on blood meal infected with 10 TCID/mL of DTMUV. Each blood-fed mosquito was isolated and allowed to lay eggs. After oviparity, the mosquitoes were tested for DTMUV infection; 43 DTMUV infected and 37 non-infected female mosquitoes with eggs were included. A total of 182 F1 progeny from DTMUV infected mosquitoes and 145 F1 progeny from non-infected mosquitoes were tested for DTMUV but all were negative. Findings indicated the potential role of Cx. tritaeniorhynchus in the DTMUV transmission cycle in duck farms in Thailand. No transovarial transmission of DTMUV was found in Cx. tritaeniorhynchus.
鸭坦布苏病毒（DTMUV）是一种新兴的鸭传染病，已在从鸭场采集的库蚊（Cx.）三带喙库蚊中检测到；然而，蚊子在泰国 DTMUV 生态中的确切作用仍不清楚。检测了三带喙库蚊和致倦库蚊对 DTMUV 的媒介能力。三带喙库蚊允许以四个水平（10、10、10 和 10 TCID/mL）的 DTMUV 进行喂食，而致倦库蚊则允许以两个水平（10 和 10 TCID/mL）的 DTMUV 进行喂食。分别以 10、10、10 和 10 TCID/mL 的 DTMUV 喂养后，三带喙库蚊的感染率分别为 1.6%、10.2%、35.8%和 59.3%，而以 10 TCID/mL 的 DTMUV 喂养后，传播和传播率分别为 20.3%和 16.9%。以 10 和 10 TCID/mL 的 DTMUV 喂养后，致倦库蚊的感染率分别为 2.5%和 2.3%，所有测试的蚊子均未发现病毒传播和传播。另一项研究旨在检测三带喙库蚊中 DTMUV 的经卵传递。允许蚊子吸食感染 10 TCID/mL 的 DTMUV 的血餐。每个吸血的蚊子都被隔离并允许产卵。产卵后，对蚊子进行 DTMUV 感染检测；共检测到 43 只 DTMUV 感染的雌性蚊子和 37 只未感染的带卵雌性蚊子。从感染 DTMUV 的蚊子中获得的 182 只 F1 后代和从未感染的蚊子中获得的 145 只 F1 后代均未检测到 DTMUV，但均为阴性。结果表明，三带喙库蚊在泰国鸭场的 DTMUV 传播循环中可能发挥作用。在三带喙库蚊中未发现 DTMUV 的经卵传递。
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