Sun Nan, Gu Yizhu, Jiang Guoxia, Wang Yuxin, Wang Pingzhi, Song Weitang, Ma Peifang, Duan Yabin, Jiao Ziyuan
College of Water Resources & Civil Engineering, China Agricultural University, Beijing, China.
Henan Pingdingshan Academy of Agricultural Sciences, Pingdingshan, China.
Front Microbiol. 2022 Jun 29;13:900779. doi: 10.3389/fmicb.2022.900779. eCollection 2022.
Endophyte resources have important research value in multiresistance breeding, ecological protection, germicide development, and other fields. In this study, high-throughput sequencing (Illumina-MiSeq) technology was employed to analyse the diversity and community composition of white radish () endophytes and rhizosphere bacteria in different compartments and cultivation conditions, including greenhouse and open field cultivation, at both the phylum and genus levels. Alpha diversity index analysis showed that the bacterial richness and diversity values of rhizosphere bacteria were higher than those of endophytes in different compartments. NMDS analysis and microbial co-occurrence network analysis showed that apart from the similarity in the endophytic bacterial composition of the leaf and root endosphere, the endophytic bacterial composition in flesh and epidermis of radish were also more similar. The dominant endophytic bacteria in white radish were Proteobacteria, Bacteroidetes, and Actinomycetes at the phylum level. We analyzed the effects of different ecological compartments and two cultivation environments on radish microorganisms, and found that ecological compartments played an important role, which was related to the mechanism of microbial assembly in plants. The same facility cultivation can also improve the diversity of radish microorganisms in different ecological compartments, and change the biomarkers that play a major role in rhizosphere microorganisms and endophytes of radish. Bacteria plays an important role in the process of plant growth, and the study of endophytes enriches the understanding of microbial diversity in white radish, which helps to provide insight into the ecological function and interaction mechanisms of plants and microorganisms.
内生菌资源在多抗性育种、生态保护、杀菌剂开发等领域具有重要的研究价值。本研究采用高通量测序（Illumina-MiSeq）技术，在门和属水平上分析了不同部位和栽培条件（包括温室和露地栽培）下白萝卜内生菌和根际细菌的多样性及群落组成。α多样性指数分析表明，不同部位根际细菌的丰富度和多样性值均高于内生菌。非度量多维尺度分析（NMDS）和微生物共现网络分析表明，除叶和根内皮层的内生细菌组成相似外，萝卜肉质和表皮中的内生细菌组成也更为相似。白萝卜中优势内生细菌在门水平上为变形菌门、拟杆菌门和放线菌门。我们分析了不同生态部位和两种栽培环境对萝卜微生物的影响，发现生态部位起重要作用，这与植物中微生物组装机制有关。相同设施栽培也可提高不同生态部位萝卜微生物的多样性，并改变在萝卜根际微生物和内生菌中起主要作用的生物标志物。细菌在植物生长过程中起重要作用，对内生菌的研究丰富了对白萝卜微生物多样性的认识，有助于深入了解植物与微生物的生态功能及相互作用机制。
