Sun Nan, Wang Yuxin, Chen Jianhua, Wang Pingzhi, Song Weitang, Ma Peifang, Duan Yabin, Jiao Ziyuan, Li Yixiao
College of Water Resources and Civil Engineering, China Agricultural University, Beijing, China.
Pingdingshan Academy of Agricultural Sciences, Pingdingshan, China.
Front Microbiol. 2022 Feb 14;13:775002. doi: 10.3389/fmicb.2022.775002. eCollection 2022.
Chinese chive has a long history of planting in China. At present, there are many studies on endophytic bacteria and rhizosphere microorganisms of Chinese chive, but the effects of ecological compartment and growth conditions on bacterial communities in Chinese chives are unclear. Here, we aimed to elucidate the differences in bacterial a-diversity, β-diversity, community structure, core species differences, interaction networks and predicted metabolic functions among bacterial communities in different ecological compartments (the phylloplane, leaf endosphere, stem endosphere, root endosphere, and rhizosphere) in Chinese chives in an open field, a solar greenhouse, an arched shed, and a hydroponic system. Sixty samples were collected from these five ecological compartments under four growth conditions, and we compared the bacterial profiles of these groups using 16S rRNA sequencing. We evaluated the differences in diversity and composition among bacterial communities in these ecological compartments, analyzed the bacterial interaction patterns under the different growth conditions, and predicted the bacterial metabolic pathways in these ecological compartments and growth conditions. The results showed that the effects of ecological compartments on bacterial diversity, community composition, interaction network pattern, and functional expression of Chinese chives were greater than those of growth condition. Ecological compartments ( = 0.5292) could better explain bacterial community division than growth conditions ( = 0.1056). The microbial interaction networks and indicator bacteria in different ecological compartments showed that most of the bacteria that played the role of key nodes (OTUs) in each ecological compartment were bacteria with high relative abundance in the compartment. However, the bacteria that played the role of key nodes (OTUs) in bulbs were not Proteobacteria with the highest relative abundance in the compartment, but Actinobacteria that were significantly enriched in the root endosphere and rhizosphere ecological compartments. In addition, interactions among bacteria were interrupted in the hydroponic system, and specific bacterial communities and interaction patterns in Chinese chives varied among growth conditions. Prediction of metabolic functions indicated that plant metabolic activity related to stress responses and induction of system resistance was greater in belowground ecological compartments.
韭菜在中国有着悠久的种植历史。目前，关于韭菜内生细菌和根际微生物的研究有很多，但生态区室和生长条件对韭菜细菌群落的影响尚不清楚。在此，我们旨在阐明不同生态区室（叶表、叶内、茎内、根内和根际）中，露天田地、日光温室、拱形棚和水培系统里韭菜细菌群落的α多样性、β多样性、群落结构、核心物种差异、相互作用网络以及预测的代谢功能的差异。在四种生长条件下，从这五个生态区室采集了60个样本，我们使用16S rRNA测序比较了这些组的细菌谱。我们评估了这些生态区室中细菌群落多样性和组成的差异，分析了不同生长条件下的细菌相互作用模式，并预测了这些生态区室和生长条件下的细菌代谢途径。结果表明，生态区室对韭菜细菌多样性、群落组成、相互作用网络模式和功能表达的影响大于生长条件。生态区室（R² = 0.5292）比生长条件（R² = 0.1056）能更好地解释细菌群落划分。不同生态区室中的微生物相互作用网络和指示细菌表明，在每个生态区室中起关键节点（OTU）作用的大多数细菌是该生态区室中相对丰度较高的细菌。然而，在鳞茎中起关键节点（OTU）作用的细菌不是该生态区室中相对丰度最高的变形菌门，而是在根内和根际生态区室中显著富集的放线菌门。此外，水培系统中细菌之间的相互作用被中断，韭菜中特定的细菌群落和相互作用模式在不同生长条件下有所不同。代谢功能预测表明，与应激反应和系统抗性诱导相关的植物代谢活性在地下生态区室中更强。
