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复发性流产女性蜕膜自然杀伤细胞中NKp46表达及细胞因子产生的评估

Evaluation of NKp46 expression and cytokine production of decidual NK cells in women with recurrent pregnancy loss.

作者信息

Yamamoto Mayu, Fukui Atsushi, Mai Chuxian, Saeki Shinichiro, Takayama Ryu, Wakimoto Yu, Yamaya Ayano, Kwak-Kim Joanne, Shibahara Hiroaki

机构信息

Department of Obstetrics and Gynecology, School of Medicine Hyogo Medical University Nishinomiya Japan.

Clinical Sciences Department, Reproductive Medicine and Immunology, Obstetrics and Gynecology, Chicago Medical School Rosalind Franklin University of Medicine and Science Vernon Hills IL USA.

出版信息

Reprod Med Biol. 2022 Jul 12;21(1):e12478. doi: 10.1002/rmb2.12478. eCollection 2022 Jan-Dec.

DOI:10.1002/rmb2.12478
PMID:35847412
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9275167/
Abstract

PURPOSE

NKp46, a receptor on NK cells, is involved in cytotoxicity and cytokine production. The authors aimed to evaluate the effect of NKp46 on decidual NK (dNK) cells during pregnancy and whether it can be a marker for immunological abnormalities in women with recurrent pregnancy loss (RPL).

METHODS

Flow-cytometric analysis was made to assess NKp46 expression and intracellular cytokine production of dNK cells. The proportion of NKp46 dNK cells was analyzed among RPL patients who aborted karyotypically normal pregnancies and those who either aborted karyotypically abnormal pregnancies or without genetic studies, and controls who were going through the induced abortion.

RESULTS

The NKp46 and %NKp46 dNK cells were significantly lower in the RPL women who aborted karyotypically normal pregnancies than in the control group. The %NKp46 dNK cells were significantly correlated with the NK1/NK2 ratio of dNK cells. The %NKp46 dNK cell cutoff for RPL with immunological abnormalities was determined by the ROC curve analysis. In women with the low %NKp46 dNK, NK1/NK2 ratios were significantly higher than those with the high.

CONCLUSION

RPL patients with an immunological abnormality have decreased NKp46 expression and NK1 shift in dNK cells. NKp46 expression could be a marker for RPL of immunological abnormalities.

摘要

目的

NKp46是自然杀伤（NK）细胞上的一种受体，参与细胞毒性作用和细胞因子的产生。作者旨在评估NKp46在孕期对蜕膜自然杀伤（dNK）细胞的影响，以及它是否可作为复发性流产（RPL）女性免疫异常的一个标志物。

方法

采用流式细胞术分析评估dNK细胞的NKp46表达及细胞内细胞因子的产生。分析了染色体核型正常妊娠流产的RPL患者、染色体核型异常妊娠流产或未进行遗传学研究的RPL患者以及人工流产对照组中NKp46⁺ dNK细胞的比例。

结果

染色体核型正常妊娠流产的RPL女性中，NKp46⁺及NKp46⁺ dNK细胞的比例显著低于对照组。NKp46⁺ dNK细胞的比例与dNK细胞的NK1/NK2比值显著相关。通过ROC曲线分析确定了免疫异常的RPL患者中NKp46⁺ dNK细胞的截断值。NKp46⁺ dNK细胞比例低的女性，其NK1/NK2比值显著高于比例高的女性。

结论

存在免疫异常的RPL患者dNK细胞中NKp46表达降低且出现NK1偏移。NKp46表达可能是免疫异常性RPL的一个标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/c17f0337ef89/RMB2-21-e12478-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/9e3900baf049/RMB2-21-e12478-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/e0990ac0ea42/RMB2-21-e12478-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/e9cef23a547d/RMB2-21-e12478-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/c17f0337ef89/RMB2-21-e12478-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/9e3900baf049/RMB2-21-e12478-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/e0990ac0ea42/RMB2-21-e12478-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/e9cef23a547d/RMB2-21-e12478-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb2/9275167/c17f0337ef89/RMB2-21-e12478-g004.jpg

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Int J Mol Sci. 2025 Apr 14;26(8):3687. doi: 10.3390/ijms26083687.
4
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