Key Lab for Agro-Product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Oxid Med Cell Longev. 2022 Jul 6;2022:9701938. doi: 10.1155/2022/9701938. eCollection 2022.
In this study, the essential oil (EO) was extracted by steam distillation from , and the extraction process was optimized by response surface methodology. The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4. Under these conditions, the theoretical yield of EO is 1.5624%. The EO compounds were analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area. The EO showed good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and reducing power. In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice. EO effectively delayed weight loss and reduced DAI score. Histological examination also observed a significant reduction in damage in the EO group. The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm). The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group ( < 0.01). SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group ( < 0.01). MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: < 0.01; M-EO: < 0.05). MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: < 0.05, H-EO: < 0.05). This study shows that EO can effectively improve the symptoms of colitis.
在这项研究中，通过水蒸气蒸馏从 中提取精油（EO），并通过响应面法优化提取工艺。最佳工艺条件如下：提取时间 4.57 小时，浸泡时间 1.33 小时，固液比 1:21.4。在此条件下，EO 的理论收率为 1.5624%。通过气相色谱-质谱联用（GC-MS）分析 EO 化合物。共检测到 52 种化学成分，其中 3-(4,8-二甲基壬-3,7-二烯基）-呋喃的含量最高，占总峰面积的 21.43%。EO 对 2,2-二苯基-1-苦基肼（DPPH）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和还原能力均表现出良好的抗氧化活性。在这项研究中，我们观察了 EO 对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）小鼠的保护作用。EO 能有效延缓体重减轻并降低 DAI 评分。组织学检查也观察到 EO 组的损伤明显减少。DSS 组小鼠的结肠长度最短，而 EO 治疗组小鼠的结肠长度长于模型组，但短于正常组（NOR：8.17±0.39cm；DSS：5.57±0.93cm；L-EO：6.47±0.78cm；M-EO：5.98±0.58cm；和 H-EO：6.1±0.52cm）。L-EO 和 SASP 组的 GSH 活性明显高于 DSS 组（<0.01）。L-EO 和 M-EO 组的 SOD 活性也明显高于 DSS 处理组（<0.01）。EO 治疗组和 SASP 组的 MDA 降低（L-EO、H-EO、SASP：<0.01；M-EO：<0.05）。EO 治疗组的 MPO 低于模型组（L-EO 组不显著，M-EO：<0.05，H-EO：<0.05）。本研究表明，EO 能有效改善结肠炎症状。
