Wang Xiao, Shu Qiang, Song Lijun, Liu Qi, Qu Xiaoxia, Li Ming
Department of Rheumatology, Qilu Hospital of Shandong University, Jinan, China.
Shandong Province Clinical Research Center for Immune Diseases and Gout, Jinan, China.
Front Med (Lausanne). 2022 Jun 30;9:915179. doi: 10.3389/fmed.2022.915179. eCollection 2022.
Despite the existing studies relating systemic lupus erythematosus (SLE) to changes in gut microbiota, the latter is affected by external factors such as diet and living environment. Herein, we compared the diversity and composition of gut microbiota in SLE patients and in their healthy family members who share the same household, to link gut microbiota, diet and SLE clinical manifestations. The study cohort included 19 patients with SLE and 19 of their healthy family members. Daily nutrition was assessed using a food frequency questionnaire (FFQ). Microbiota was analyzed using amplicons from the V4 regions of the 16S rRNA gene, to obtain microbiota diversity, taxa relative abundances and network analysis. The gut microbiota in the SLE group had lower alpha diversity and higher heterogeneity than the control group. SLE patients had decreased Acidobacteria, Gemmatimonadetes, Nitrospirae and Planctomycetes at the phylum level, and increased , and at the genus level. was extremely enriched among patients with lupus nephritis. and OTUs were associated with diet and clinical features of SLE. Finally, the gut microbiota of SLE patients remained different from that in healthy controls even after accounting for living conditions and diet.
尽管现有研究将系统性红斑狼疮（SLE）与肠道微生物群的变化联系起来，但后者会受到饮食和生活环境等外部因素的影响。在此，我们比较了SLE患者及其同住的健康家庭成员的肠道微生物群的多样性和组成，以关联肠道微生物群、饮食和SLE临床表现。研究队列包括19名SLE患者及其19名健康家庭成员。使用食物频率问卷（FFQ）评估日常营养状况。使用来自16S rRNA基因V4区域的扩增子分析微生物群，以获得微生物群多样性、分类群相对丰度和网络分析结果。SLE组的肠道微生物群与对照组相比，α多样性较低，异质性较高。SLE患者在门水平上放线菌门、芽单胞菌门、硝化螺旋菌门和浮霉菌门减少，在属水平上、和增加。在狼疮性肾炎患者中极度富集。和OTU与SLE的饮食和临床特征相关。最后，即使在考虑生活条件和饮食因素后，SLE患者的肠道微生物群仍与健康对照组不同。
