Department of Nephrology, The First People's Hospital of Foshan, Guangdong Province, Foshan 528000, China.
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Harvard University, Boston, Massachusetts 02115, USA.
Biomed Res Int. 2021 Feb 18;2021:8854969. doi: 10.1155/2021/8854969. eCollection 2021.
Increasing evidences have reported gut microbiota dysbiosis in many diseases, including chronic kidney disease and pediatric idiopathic nephrotic syndrome (INS). There is lack evidence of intestinal microbiota dysbiosis in adults with INS, however. Here, we to address the association between the gut microbiome and INS.
Stool samples of 35 adult INS patients and 35 healthy volunteers were collected. Total bacterial DNA was extracted, and the V4 regions of the bacterial 16S ribosomal RNA gene were sequenced. The fecal microbiome was analyzed using bioinformatics. The correlation analysis between altered taxa and clinical parameters was also included.
We found that microbial diversity in the gut was reduced in adult patients with INS. , , , , , , , , , , , , and were significantly reduced, while , , , , , and were markedly increased in patients with INS. In addition, , , and were negatively correlated with serum creatinine. Blood urea nitrogen levels were positively correlated with , , , , and , but were negatively correlated with and . , , , , , and were positively correlated with albumin. Proteinuria was positively correlated with , , , , , , , and , but was negatively correlated with , , and e.
Our findings show compositional alterations of intestinal microbiota in adult patients with INS and correlations between significantly altered taxa and clinical parameters, which points out the direction for the development of new diagnostics and therapeutic approaches targeted intestinal microbiota.
越来越多的证据表明，肠道微生物失调与许多疾病有关，包括慢性肾脏病和儿童特发性肾病综合征（INS）。然而，成人 INS 患者肠道微生物失调的证据不足。在这里，我们旨在探讨肠道微生物群与 INS 之间的关联。
收集 35 例成人 INS 患者和 35 名健康志愿者的粪便样本。提取总细菌 DNA，并对细菌 16S 核糖体 RNA 基因的 V4 区进行测序。使用生物信息学分析粪便微生物群。还包括改变的分类群与临床参数之间的相关性分析。
我们发现，成人 INS 患者肠道微生物多样性降低。、、、、、、、、、、和减少，而 INS 患者中显著增加。此外，和与血清肌酐呈负相关。血尿素氮水平与、、、、和呈正相关，但与和呈负相关。、、、、、和与白蛋白呈正相关。蛋白尿与、、、、、、和呈正相关，但与和呈负相关。
我们的研究结果表明，成人 INS 患者肠道微生物群存在组成改变，并且显著改变的分类群与临床参数之间存在相关性，这为开发针对肠道微生物群的新诊断和治疗方法指明了方向。
