Akbari Fatemeh, Azadbakht Mohammad, Gaurav Anand, Azimi Fatemeh, Mahdizadeh Zahra, Vahedi Lale, Barzegar Nejad Ayob, Chabra Aroona, Eghbali Mohammad
Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Faculty of Pharmaceutical Sciences, UCSI University, Kuala Lumpur, Malaysia.
Adv Urol. 2022 Jul 5;2022:5742431. doi: 10.1155/2022/5742431. eCollection 2022.
Benign prostatic hyperplasia (BPH) is a common disease that affects elderly men with various complications. This study evaluates the effects of an Iranian traditional herbal medicine "Atrifil and Oshagh gum" on BPH in male Wistar rats. is a combination of three medicinal plants: " extracts, and gum is D. Dono gum. In this study, 30 male Wistar rats were divided into five groups: normal control, disease, finasteride, and extract with 300 and 600 mg/kg groups. The extract is a combination of hydroalcoholic extract and gum. All groups received intramuscular testosterone enanthate to induce BPH except the normal control group. On the twenty-eighth day, prostate glands were separated. Histopathological changes were observed. Furthermore, the prostate-specific antigen (PSA) and prostate weights were measured. The binding propensities of finasteride, equol, and flavonoids present in this extract such as quercetin, rutin, and kaempferol for 5-reductase, estrogen receptor alpha and beta, and estrogen-related receptor gamma were assessed using in silico docking approach. Histopathological evaluation, biochemical parameter, and PSA level results indicated significant inhibition of accruing and progression of BPH in groups treated with 600 mg/kg extract ( < 0.01). Furthermore, molecular docking showed that rutin had a high affinity to bind the receptors 5-reductase, estrogen receptor beta, and estrogen-related receptor gamma even more than finasteride, and on average, quercetin had a higher affinity to all these receptors. In the end, it can be concluded that and gum is effective in preventing BPH.
良性前列腺增生（BPH）是一种影响老年男性且伴有多种并发症的常见疾病。本研究评估了一种伊朗传统草药“Atrifil和奥沙格树胶”对雄性Wistar大鼠良性前列腺增生的影响。它是三种药用植物的组合：提取物，并且树胶是D.多诺树胶。在本研究中，30只雄性Wistar大鼠被分为五组：正常对照组、疾病组、非那雄胺组以及提取物300和600mg/kg组。提取物是水醇提取物和树胶的组合。除正常对照组外，所有组均接受肌肉注射庚酸睾酮以诱导良性前列腺增生。在第28天，分离前列腺。观察组织病理学变化。此外，测量前列腺特异性抗原（PSA）和前列腺重量。使用计算机对接方法评估非那雄胺、雌马酚以及该提取物中存在的黄酮类化合物（如槲皮素、芦丁和山奈酚）对5-还原酶、雌激素受体α和β以及雌激素相关受体γ的结合倾向。组织病理学评估、生化参数和PSA水平结果表明，600mg/kg提取物处理组中良性前列腺增生的发生和进展受到显著抑制（P<0.01）。此外，分子对接显示芦丁与5-还原酶、雌激素受体β和雌激素相关受体γ的结合亲和力甚至高于非那雄胺，并且平均而言，槲皮素对所有这些受体具有更高的亲和力。最后，可以得出结论，Atrifil和奥沙格树胶对预防良性前列腺增生有效。
