Department of Pediatrics, Gastroenterology Division, King Saud University Medical City, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Division of Pediatric Gastroenterology, Children's Specialized Hospital, King Fahad Medical City; Faculty of Medicine, AlFaisal University, Riyadh, Kingdom of Saudi Arabia.
Saudi J Gastroenterol. 2022 Jul-Aug;28(4):312-317. doi: 10.4103/sjg.sjg_585_21.
Knowledge of microbiota in health is essential for clinical research on the role of microbiota in disease. We aimed to characterize the intestinal microbiota in healthy Saudi children.
In this community-based study, stool samples were collected from a randomly selected sample of 20 healthy school children of Saudi origin. The samples were frozen at -80°C till analysis. Bacterial DNA was isolated and libraries were prepared using the Illumina Nextera XT library preparation kit. Unassembled sequencing reads were directly analyzed and quantified for each organism's relative abundance. The abundance for each organism was calculated and expressed as the average relative percentage from phyla to species.
The median age was 11.3 (range 6.8-15.4) years, and 35% of them were males. The three most abundant phyla were Firmicutes, Bacteroidetes, and Actinobacteria accounting for 49%, 26%, and 24%, respectively. The most abundant genera included Bifidobacterium, Bacteroides, and Blautia accounting for 18.9%, 12.8%, and 8.2%, respectively. Finally, the most abundant species included 14 species belonging to the genus Bacteroides and nine species belonging to Bifidobacterium.
The abundance of intestinal microbiome in healthy Saudi children is different from that of other populations. Further studies are needed to understand the causes of variation between populations, which might lead to new preventive methods and treatment strategies of diseases caused by microbial dysbiosis.
了解健康人群中的微生物群对于研究微生物群在疾病中的作用的临床研究至关重要。本研究旨在描述沙特健康儿童的肠道微生物群。
在这项基于社区的研究中，我们从沙特籍 20 名健康在校儿童中随机抽取粪便样本。将样本在-80°C 下冷冻直至分析。使用 Illumina Nextera XT 文库制备试剂盒分离细菌 DNA 并制备文库。直接对未组装的测序reads 进行分析，并对每个生物体的相对丰度进行定量。计算每个生物体的丰度，并以从门到种的平均相对百分比表示。
中位年龄为 11.3 岁（范围 6.8-15.4 岁），其中 35%为男性。最丰富的三个菌门是厚壁菌门、拟杆菌门和放线菌门，分别占 49%、26%和 24%。最丰富的属包括双歧杆菌属、拟杆菌属和布劳特氏菌属，分别占 18.9%、12.8%和 8.2%。最后，最丰富的物种包括 14 种属于拟杆菌属的物种和 9 种属于双歧杆菌属的物种。
健康沙特儿童肠道微生物群的丰度与其他人群不同。需要进一步研究以了解人群之间变异的原因，这可能会导致针对由微生物失调引起的疾病的新预防方法和治疗策略。
