Department of General Surgery, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China.
2 Institute of Chinese Medicine Surgery, Anhui Academy of Traditional Chinese Medicine, Hefei 230031, China.
J Tradit Chin Med. 2022 Aug;42(4):556-564. doi: 10.19852/j.cnki.jtcm.20220519.004.
To investigate the influence of Qihuang decoction on enteric nervous system after gastrectomy in rats.
The morphology, distribution and number of intestinal neurons in enteric nervous system (ENS) were observed by immunofluorescence labeling and confocal laser scanning microscopy. Reverse transcription-polymerase chain reaction and Western blot were used to detect the mRNA and protein expression of intestinal neurotransmitters and corresponding receptors in ENS.
The morphology and distribution of enteric neurons in ENS were changed after gastrectomy, and these neurons in Qihuang decoction group were similar with that of sham operation group. The number of ACh and SP positive neurons, mRNA and protein expression of excitatory neurotransmitters (AChE, SP) and receptors (M3R, NK1R) were decreased after gastrectomy. And the intervention of Qihuang decoction could increase the number of ACh and SP positive neurons and promote the expression of their mRNA and protein. For vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS), the number of neurons and mRNA and protein expression of inhibitory neurotransmitters (VIP and NOS) and receptors (VIP2R) were increased after gastrectomy. And these rising indexes fall back after the intervention of Qihuang decoction. Besides, the intestinal propulsion rate in QH group was significantly increased than that in SEN and IEN group.
These experimental results showed that after gastrectomy, early intervention with Qihuang decoction in small intestine will contribute to the postoperative recovery of enteric nervous system and intestinal propulsion rate, and consequently enhance gastrointestinal motility.
探讨芪黄汤对胃切除术后大鼠肠神经系统的影响。
采用免疫荧光标记和共聚焦激光扫描显微镜观察肠神经系统（ENS）中肠道神经元的形态、分布和数量。采用逆转录-聚合酶链反应和 Western blot 检测 ENS 中肠道神经递质及其相应受体的 mRNA 和蛋白表达。
胃切除术后 ENS 中肠神经元的形态和分布发生改变，芪黄汤组的这些神经元与假手术组相似。胃切除术后，ACh 和 SP 阳性神经元数量、兴奋性神经递质（AChE、SP）及其受体（M3R、NK1R）的 mRNA 和蛋白表达减少，芪黄汤的干预可增加 ACh 和 SP 阳性神经元的数量，并促进其 mRNA 和蛋白的表达。对于血管活性肠肽（VIP）和一氧化氮合酶（NOS），抑制性神经递质（VIP 和 NOS）及其受体（VIP2R）的神经元数量和 mRNA 及蛋白表达在胃切除术后增加。而这些上升指标在芪黄汤干预后回落。此外，QH 组的肠道推进率明显高于 SEN 和 IEN 组。
这些实验结果表明，胃切除术后早期干预小肠芪黄汤有助于肠神经系统和肠道推进率的术后恢复，从而增强胃肠动力。
