Kruger T F, Stander F S, Smith K, Van der Merwe J P, Lombard C J
J In Vitro Fert Embryo Transf. 1987 Feb;4(1):10-2. doi: 10.1007/BF01555428.
The effect of serum supplementation was evaluated on 108 oocytes obtained from 29 patients. The oocytes were all mature and randomly distributed in two groups. Each ovum was paired with another ovum within the same patient. The experimental group contained no serum in the insemination and growth medium, but in the control group 10% serum was added to the insemination and growth medium. In the experimental group the cleavage rate was 66.7%, compared to 83.3% in the control group. The embryos from each patient were individually evaluated. The sign test was used for statistical analysis. A significant difference between the two groups (P = 0.01) indicated that in this study the addition of serum to the medium gives better cleavage results. The effect of serum on embryos is discussed, as well as the practical implications of this finding.
对从29名患者获取的108枚卵母细胞评估血清添加的效果。这些卵母细胞均成熟且随机分为两组。同一患者的每个卵子与另一个卵子配对。实验组的授精和生长培养基中不添加血清，而对照组的授精和生长培养基中添加10%血清。实验组的卵裂率为66.7%，而对照组为83.3%。对每位患者的胚胎进行单独评估。采用符号检验进行统计分析。两组之间的显著差异（P = 0.01）表明，在本研究中向培养基中添加血清可获得更好的卵裂结果。讨论了血清对胚胎的影响以及这一发现的实际意义。
