School of Life Sciences, Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Institute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Sci Data. 2022 Jul 18;9(1):418. doi: 10.1038/s41597-022-01470-8.
Anastasis is a cell recovery mechanism that rescues dying cells from the brink of death. Reversal of apoptosis is the first example of anastasis. Here, we describe a comprehensive dataset containing time-course mRNA expression profiles for reversal of ethanol-induced apoptosis in mouse primary liver cells in νitro. This transcriptome dataset includes the conditions of the untreated cells, cells undergoing apoptosis triggered by incubating with cell death inducer of 4.5% ethanol for 5 hours, and apoptosis reversal of ethanol-induced cells at the early (3 hour), middle (6 hour), and late (24, 48 hour) stages after being washed with and incubated in fresh cell culture medium. By comparing this dataset with the transcriptomic profiles of other anastasis models generated with different combinations of cell types and cell death inducers, investigators can identify the key regulators governing reversal of apoptosis and other reversible cell death processes. Therefore, reusing or reanalysing this dataset will facilitate the future studies on the physiological, pathological, and therapeutic implications of anastasis.
复苏是一种细胞恢复机制，可将濒死的细胞从死亡边缘拯救回来。细胞凋亡的逆转是复苏的第一个例子。在这里，我们描述了一个综合数据集，其中包含了体外培养的小鼠原代肝细胞中乙醇诱导的细胞凋亡逆转的时间过程 mRNA 表达谱。该转录组数据集包括未处理细胞的条件、用 4.5%乙醇孵育 5 小时引发凋亡的细胞以及用新鲜细胞培养基洗涤和孵育后在早期（3 小时）、中期（6 小时）和晚期（24、48 小时）阶段逆转乙醇诱导的细胞凋亡的条件。通过将这个数据集与使用不同细胞类型和细胞死亡诱导剂组合生成的其他复苏模型的转录组谱进行比较，研究人员可以鉴定出调控细胞凋亡逆转和其他可逆性细胞死亡过程的关键调节因子。因此，重新使用或重新分析这个数据集将有助于未来对复苏的生理、病理和治疗意义的研究。
