Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.
Research Centre for Physical Activity and Health LIKES, Jyväskylä, Finland.
Physiol Rep. 2022 Jul;10(14):e15394. doi: 10.14814/phy2.15394.
Little is known how acute exercise-induced inflammation and metabolic stress affect immune cell bioenergetics and the portion of its components. Therefore, we investigated acute effects of eccentric-only (E), concentric-only (C) and combined eccentric-concentric resistance exercise (E + C) bouts on cellular respiration of peripheral blood mononuclear cells (PBMCs). Twelve strength-trained young men performed bench press resistance exercises in randomized order. Venous blood samples were drawn at pre-, 5 min post- and 24 h post-exercise. Several PBMC respiration states were measured using high-resolution respirometry. Levels of leukocytes, interleukin 6 (IL-6), C-reactive protein (CRP), creatine kinase (CK), blood lactate and maximum voluntary isometric force were measured from the same time points. Effects of blood lactate and pH change on bioenergetics of PBMCs were investigated ex vivo. PBMC routine respiration (p = 0.017), free routine capacity (p = 0.025) and ET-capacity (p = 0.038) decreased immediately after E + C. E responded in opposite manner 5 min post-exercise compared to E + C (p = 0.013) and C (p = 0.032) in routine respiration, and to E + C in free routine activity (p = 0.013). E + C > C > E was observed for increased lactate levels and decreased isometric force that correlated with routine respiration (R = -0.369, p = 0.035; R = 0.352, p = 0.048). Lactate and pH change did not affect bioenergetics of PBMCs. Acute resistance exercise affected cellular respiration of PBMCs, with training volume and the amount of metabolic stress appear influential. Results suggest that acute inflammation response does not contribute to changes seen in cellular respiration, but the level of peripheral muscle fatigue and metabolic stress could be explaining factors.
目前尚不清楚急性运动引起的炎症和代谢应激如何影响免疫细胞的生物能量及其组成部分。因此，我们研究了单纯离心（E）、单纯向心（C）和离心-向心混合抗阻运动（E+C）对周围血单核细胞（PBMC）细胞呼吸的急性影响。12 名力量训练的年轻男性以随机顺序进行卧推抗阻运动。在运动前、运动后 5 分钟和 24 小时采集静脉血样本。使用高分辨率呼吸仪测量 PBMC 的几种呼吸状态。在相同时间点测量白细胞、白细胞介素 6（IL-6）、C 反应蛋白（CRP）、肌酸激酶（CK）、血乳酸和最大自主等长力量。研究了体外血乳酸和 pH 值变化对 PBMC 生物能量学的影响。E+C 后即刻，PBMC 常规呼吸（p=0.017）、自由常规能力（p=0.025）和 ET 能力（p=0.038）降低。E 在运动后 5 分钟的反应与 E+C（p=0.013）和 C（p=0.032）的常规呼吸相反，与 E+C 的自由常规活动相反（p=0.013）。观察到 E+C>C>E 的乳酸水平升高和等长力量降低，与常规呼吸相关（R=-0.369，p=0.035；R=0.352，p=0.048）。乳酸和 pH 值变化不影响 PBMC 的生物能量学。急性抗阻运动影响 PBMC 的细胞呼吸，训练量和代谢应激量似乎有影响。结果表明，急性炎症反应不会导致细胞呼吸发生变化，但外周肌肉疲劳和代谢应激水平可能是解释因素。
