Department of Animal Morphology and Physiology, Federal Rural of University of Pernambuco, Recife Pernambuco, Brazil.
Department of Pathology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Arch Microbiol. 2022 Jul 19;204(8):503. doi: 10.1007/s00203-022-03134-y.
Thrombosis is a hematological disorder characterized by the formation of intravascular thrombi, which contributes to the development of cardiovascular diseases. Fibrinolytic enzymes are proteases that promote the hydrolysis of fibrin, promoting the dissolution of thrombi, contributing to the maintenance of adequate blood flow. The characterization of new effective, safe and low-cost fibrinolytic agents is an important strategy for the prevention and treatment of thrombosis. However, the development of new fibrinolytics requires the use of complex methodologies for purification, physicochemical characterization and evaluation of the action potential and toxicity of these enzymes. In this context, microbial enzymes produced by bacteria of the Bacillus genus are promising and widely researched sources to produce new fibrinolytics, with high thrombolytic potential and reduced toxicity. Thus, this review aims to provide a current and comprehensive understanding of the different Bacillus species used for the production of fibrinolytic proteases, highlighting the purification techniques, biochemical characteristics, enzymatic activity and toxicological evaluations used.
血栓形成是一种以血管内血栓形成特征的血液学疾病，导致心血管疾病的发展。纤维蛋白溶解酶是促进纤维蛋白水解的蛋白酶，促进血栓溶解，有助于维持足够的血流。鉴定新的有效、安全和低成本的纤维蛋白溶解剂是预防和治疗血栓形成的重要策略。然而，新的纤维蛋白溶解剂的开发需要使用复杂的方法来纯化、理化特性分析以及评估这些酶的作用潜力和毒性。在这种情况下，由芽孢杆菌属细菌产生的微生物酶是有前途的、广泛研究的新型纤维蛋白溶解酶的来源，具有高溶栓潜力和降低的毒性。因此，本综述旨在提供对用于生产纤维蛋白溶解蛋白酶的不同芽孢杆菌属物种的当前和全面的理解，重点介绍所使用的纯化技术、生化特性、酶活性和毒理学评估。
