SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No.8 Futong Dongdajie, Wangjing, Chaoyang District, Beijing 100102, China.
Molecules. 2012 Oct 19;17(10):12297-311. doi: 10.3390/molecules171012297.
Interest in the antioxidant activity of bamboo leaves is growing. To discover new sources of natural antioxidants, a TLC bioautography method combined with a new image processing method was developed to evaluate the antioxidant activity of leaf extracts from 15 different species of bamboo. The results showed that the methanolic extract of Bambusa. textilis McClure possessed the highest antioxidant activity among the selected bamboo species. To rapidly identify the antioxidant compounds, the crude extract of B. textilis McClure was analysed by HPLC-UV, and HPLC-micro-fractionation of the extract was carried out. Based on TLC bioautography-guided fractionation, three antioxidant fractions were isolated from B. textilis McClure by preparative chromatography. These three antioxidant compounds were identified as isoorientin 4''-O β-D-xylopyranoside, isoorientin 2''-O-α-L-rhamnoside and isoorientin according to their UV, MS, and NMR data. The proposed TLC screening method could therefore be an easy way to evaluate the antioxidant activity of bamboo leaves, and the results achieved should prove very helpful for promoting their utilization, as B. textilis McClure can be considered a promising plant source of natural antioxidants.
竹叶的抗氧化活性引起了越来越多的关注。为了发现新的天然抗氧化剂来源，开发了一种 TLC 生物自显影方法结合新的图像处理方法，以评估来自 15 种不同竹种的叶提取物的抗氧化活性。结果表明，在所选竹种中，慈竹的甲醇提取物具有最高的抗氧化活性。为了快速鉴定抗氧化化合物，对慈竹的粗提取物进行了 HPLC-UV 分析，并对提取物进行了 HPLC 微分级分离。根据 TLC 生物自显影引导的分级，通过制备色谱从慈竹中分离出三种抗氧化成分。根据它们的 UV、MS 和 NMR 数据，这三种抗氧化化合物被鉴定为异荭草苷 4''-O-β-D-吡喃木糖基、异荭草苷 2''-O-α-L-鼠李糖苷和异荭草苷。因此，所提出的 TLC 筛选方法可以成为评估竹叶抗氧化活性的一种简单方法，并且所获得的结果应该对促进它们的利用非常有帮助，因为慈竹可以被认为是一种很有前途的天然抗氧化剂植物来源。
