School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
Biomaterials and Tissue Engineering Research Group, Department of Interdisciplinary Technologies, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran.
Sci Rep. 2022 Jul 19;12(1):12336. doi: 10.1038/s41598-022-16568-7.
Graphene and its family have a great potential in tissue engineering because of their super mechanical properties, electrical conductivity and antibacterial properties. Considering other properties of graphene such as high surface area and ready-to-use functionalization according to the high oxygen-containing groups in graphene oxide family, some needs could be addressed in bone tissue engineering. Herein, we synthesized and decorated strontium nanoparticles (SrNPs) during the reduction process of graphene oxide using green and novel method. Without using hydrazine or chemical linkers, strontium NPs were synthesized and decorated on the surface of rGO simultaneously using BSA. The results of the UV-Vis, FTIR and Raman spectroscopy demonstrated that BSA could successfully reduce graphene oxide and decorated SrNPs on the surface of rGO. FESEM and TEM exhibited that in situ synthesized SrNPs had 25-30 nm diameter. Interestingly, cell viability for MC3T3-E1 cells treated with SrNPs-rGO, were significantly higher than BSA-rGO and GO in constant concentration. Furthermore, we investigated the alkaline phosphatase activity (ALP) of these nanosheets that the results demonstrated Sr-BSA-rGO enhanced ALP activity more than GO and BSA-rGO. Remarkably, the relative expression of RUNX 2 and Col1 genes of MC3T3-E1 cells was boosted when treated with Sr-BSA-rGO nanosheets. This study revealed that using proteins and other biomolecules as green and facile agent for decoration of smart nanoparticles on the surface of nanosheets, would be promising and assist researcher to replace the harsh and toxic hydrazine like materials with bio-friendly method. These results demonstrated that Sr-BSA-rGO had the excellent capability for regenerating bone tissue and could be used as an osteogenesis booster in implants.
石墨烯及其家族由于具有超机械性能、导电性和抗菌性能，在组织工程中有很大的应用潜力。考虑到石墨烯的其他一些性质，如高比表面积和根据氧化石墨烯家族中高含氧基团易于功能化，在骨组织工程中可以解决一些需求。在此，我们采用绿色、新颖的方法，在氧化石墨烯的还原过程中合成和修饰了锶纳米粒子（SrNPs）。没有使用水合肼或化学连接剂，BSA 同时将 SrNPs 合成并修饰在 rGO 的表面上。UV-Vis、FTIR 和 Raman 光谱的结果表明，BSA 可以成功还原氧化石墨烯并在 rGO 表面上修饰 SrNPs。FESEM 和 TEM 显示，原位合成的 SrNPs 直径为 25-30nm。有趣的是，SrNPs-rGO 处理的 MC3T3-E1 细胞的细胞活力明显高于恒定浓度下的 BSA-rGO 和 GO。此外，我们研究了这些纳米片的碱性磷酸酶活性（ALP），结果表明 Sr-BSA-rGO 比 GO 和 BSA-rGO 更能增强 ALP 活性。值得注意的是，当用 Sr-BSA-rGO 纳米片处理时，MC3T3-E1 细胞的 RUNX2 和 Col1 基因的相对表达量增加。这项研究表明，使用蛋白质和其他生物分子作为绿色简便的试剂，将智能纳米粒子修饰在纳米片的表面上，将是很有前途的，并有助于研究人员用生物友好的方法取代苛刻和有毒的水合肼类材料。这些结果表明，Sr-BSA-rGO 具有出色的骨组织再生能力，可以用作植入物中的成骨增强剂。
