Department of Biochemistry and Molecular Biology, Luzhou, China.
Department of Physiology, College of Basic Medical Sciences, Southwest Medical University, Luzhou, China.
Bull Exp Biol Med. 2022 Jul;173(3):366-370. doi: 10.1007/s10517-022-05550-y. Epub 2022 Jul 20.
Baicalin (naturally bioactive flavone compound isolated from Scutellaria baicalensis) has been demonstrated to exert strong anticancer activity against various tumor cells. However, the possibility of using baicalin for the treatment of cholangiocarcinoma and its effectiveness remain unstudied. The effect of baicalin on QBC939 cholangiocarcinoma cell culture was studied by assessing cell viability (CCK-8 test) and expression of the key proteins (Western blotting). Baicalin induced apoptosis of QBC939 cells in culture in a dose- and time-dependent manner. The proapoptotic effect was attributed to inhibition of the mTORC1-p70S6K signaling pathway resulting from baicalin-induced AMPK activation. These findings provide a new approach for cholangiocarcinoma treatment and serve as a basis for developing baicalin-based combination cancer therapy strategies.
黄芩苷（从黄芩中分离得到的天然生物活性黄酮类化合物）已被证明对各种肿瘤细胞具有强烈的抗癌活性。然而，将黄芩苷用于治疗胆管癌及其疗效尚未得到研究。本研究通过评估细胞活力（CCK-8 试验）和关键蛋白的表达（Western blot）来研究黄芩苷对 QBC939 胆管癌细胞培养的影响。黄芩苷以剂量和时间依赖的方式诱导培养的 QBC939 细胞凋亡。促凋亡作用归因于黄芩苷诱导的 AMPK 激活导致 mTORC1-p70S6K 信号通路的抑制。这些发现为胆管癌的治疗提供了一种新的方法，并为开发基于黄芩苷的联合癌症治疗策略奠定了基础。
