Xia Yingchen, Zha Jianhua, Curull Víctor, Sánchez-Font Albert, Guitart Maria, Rodríguez-Fuster Alberto, Aguiló Rafael, Barreiro Esther
Pulmonology Dept, Lung Cancer and Muscle Research Group, Hospital del Mar-IMIM, Parc de Salut Mar, Dept of Medicine and Life Sciences (MELIS), Universitat Pompeu Fabra (UPF), Medical School, Universitat Autònoma de Barcelona, Parc de Recerca Biomèdica de Barcelona (PRBB), Barcelona, Spain.
Dept of Thoracic Surgery, The first Affiliated Hospital of Nanchang University, Nanchang, China.
ERJ Open Res. 2022 Jul 18;8(3). doi: 10.1183/23120541.00105-2022. eCollection 2022 Jul.
Epithelial-mesenchymal transition (EMT) is involved in the pathophysiology of lung cancer (LC) and COPD, and the latter is an important risk factor for LC. We hypothesised that the EMT gene expression profile and signalling cascade may differ in LC patients with COPD from those with no respiratory diseases. In lung tumour specimens obtained through video-assisted thoracoscopic surgery from LC (n=20, control group) and LC-COPD patients (n=30), gene expression (quantitative real-time PCR amplification) of EMT markers , , , , , , , , and was detected. In lung tumours of LC-COPD compared to LC patients, gene expression of , , and significantly declined, while no significant differences were detected for the other analysed markers. A significant correlation was found between pack-years (smoking burden) and gene expression among LC-COPD patients. LC-COPD patients exhibited mild-to-moderate airway obstruction and a significant reduction in diffusion capacity compared to LC patients. In lung tumour samples of patients with COPD, several markers of EMT expression, namely , , and , were differentially expressed suggesting that these markers are likely to play a role in the regulation of EMT in patients with this respiratory disease. Cigarette smoke did not seem to influence the expression of EMT markers in this study. These results have potential clinical implications in the management of patients with LC, particularly in those with underlying respiratory diseases.
上皮-间质转化（EMT）参与肺癌（LC）和慢性阻塞性肺疾病（COPD）的病理生理学过程，而后者是肺癌的一个重要危险因素。我们假设，患有COPD的LC患者与无呼吸系统疾病的LC患者相比，其EMT基因表达谱和信号级联可能存在差异。在通过电视辅助胸腔镜手术获取的LC患者（n = 20，对照组）和LC-COPD患者（n = 30）的肺肿瘤标本中，检测了EMT标志物、、、、、、、、和的基因表达（定量实时PCR扩增）。与LC患者相比，LC-COPD患者肺肿瘤中的、、和基因表达显著下降，而其他分析标志物未检测到显著差异。在LC-COPD患者中，吸烟包年数（吸烟负担）与基因表达之间存在显著相关性。与LC患者相比，LC-COPD患者表现出轻度至中度气道阻塞，弥散能力显著降低。在COPD患者的肺肿瘤样本中，几种EMT表达标志物，即、、和，存在差异表达，表明这些标志物可能在这种呼吸系统疾病患者的EMT调节中发挥作用。在本研究中，香烟烟雾似乎并未影响EMT标志物的表达。这些结果对LC患者，尤其是患有潜在呼吸系统疾病的患者的管理具有潜在的临床意义。
