Departments of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Departments of Parasitology, Benha National University (BNU), Qalyubia, Egypt.
Departments of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Exp Parasitol. 2024 Oct;265:108823. doi: 10.1016/j.exppara.2024.108823. Epub 2024 Aug 24.
Trichinosis is a common parasitic disease that affects the striated skeletal muscles, causing apoptotic and degenerative changes associated with myogenin expression in the affected myocytes. Hence, this study aimed to assess the ameliorative effects of stem cells and atorvastatin added to ivermectin on the infected myocytes during the muscular phase of murine trichinosis. 120 laboratory Swiss albino male mice were divided into 10 groups, and each group was subdivided into intestinal and muscular phases (each n = 6); uninfected control; untreated infected control; infected received ivermectin monotherapy; infected received atorvastatin monotherapy; infected received stem cells monotherapy; infected received ivermectin and atorvastatin dual therapy; infected received ivermectin and stem cells dual therapy; infected received atorvastatin and stem cells dual therapy; infected received ivermectin 0.2, atorvastatin 40, and stem cells triple therapy; and infected received ivermectin 0.1, atorvastatin 20, and stem cells triple therapy. Intestinal phase mice were sacrificed on the 5th day post-infection, while those of the muscular phase were sacrificed on the 35th day post-infection. Parasitological, histopathological, ultrastructural, histochemical, biochemical, and myogenin gene expression assessments were performed. The results revealed that mice that received ivermectin, atorvastatin, and stem cell triple therapies showed the maximum reduction in the adult worm and larvae burden, marked improvement in the underlying muscular degenerative changes (as was noticed by histopathological, ultrastructural, and histochemical Feulgen stain assessment), lower biochemical levels of serum NK-κB and tissue NO, and lower myogenin expression. Accordingly, the combination of stem cells, atorvastatin, and ivermectin affords a potential synergistic activity against trichinosis with considerable healing of the underlying degenerative sequel.
旋毛虫病是一种常见的寄生虫病，影响横纹肌，导致受影响的肌细胞中与肌生成素表达相关的凋亡和退行性变化。因此，本研究旨在评估干细胞和阿托伐他汀联合依维菌素对感染肌细胞在旋毛虫病肌肉期的改善作用。将 120 只实验室瑞士白化雄性小鼠分为 10 组，每组再分为肠期和肌肉期（每组 n=6）；未感染对照组；未治疗感染对照组；感染组给予伊维菌素单一疗法；感染组给予阿托伐他汀单一疗法；感染组给予干细胞单一疗法；感染组给予伊维菌素和阿托伐他汀双重疗法；感染组给予伊维菌素和干细胞双重疗法；感染组给予阿托伐他汀和干细胞双重疗法；感染组给予伊维菌素 0.2、阿托伐他汀 40 和干细胞三重疗法；感染组给予伊维菌素 0.1、阿托伐他汀 20 和干细胞三重疗法。肠期小鼠于感染后第 5 天处死，肌肉期小鼠于感染后第 35 天处死。进行寄生虫学、组织病理学、超微结构、组织化学、生物化学和肌生成素基因表达评估。结果显示，接受伊维菌素、阿托伐他汀和干细胞三重疗法的小鼠成虫和幼虫负荷减少最多，肌肉退行性变化明显改善（组织病理学、超微结构和组织化学 Feulgen 染色评估），血清 NK-κB 和组织 NO 的生化水平较低，肌生成素表达较低。因此，干细胞、阿托伐他汀和伊维菌素的联合应用对旋毛虫病具有潜在的协同作用，并能显著改善潜在的退行性后遗症。
