Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of Chinese Medicine, Changchun, 130117, China.
Department of Marine Life Science, Jeju National University, Jeju, 63243, Republic of Korea.
Chem Biodivers. 2022 Sep;19(9):e202200495. doi: 10.1002/cbdv.202200495. Epub 2022 Aug 16.
Edible Brown Seaweed Sargassum fusiforme (Harvey) Setchell, 1931 abbreviated as Sargassum fusiforme was used for folk medical therapy in East Asia countries over five hundred years. Saringosterol acetate (SA) was isolated from S. fusiforme in our previous study and indicated various effects. However, anti-obesity activity of SA and its mechanism still unknown.
The inhibitory effect of SA, isolated from S. fusiforme, on adipogenesis in 3T3-L1 adipocytes was investigated in vitro and in zebrafish model. Cell toxicity, differentiation, signaling pathway, and lipid accumulation of SA treated 3T3-L1 adipocytes were determined. The body weight and triglyceride content of diet-induced obese (DIO) adult male zebrafish were measured from 12 to 17 weeks after fertilization.
SA attenuated the differentiation of cells and reduced lipid accumulation, and triglyceride content in the 3T3-L1 adipocytes. During the differentiation of adipocytes, SA suppressed fat accumulation and decreased the expression of signal factors responsible for adipogenesis. In SA-treated adipocytes, while fatty acid synthetase was downregulated, AMP-activated protein kinase (AMPK) was upregulated. Furthermore, SA suppressed body weight and triglyceride content in DIO zebrafish.
SA is a potential therapeutic agent in the management of metabolic disorders, such as obesity.
食用褐藻马尾藻（Harvey）Setchell，1931 年简称马尾藻已在东亚国家被用于民间医学治疗五百多年。我们之前的研究从马尾藻中分离出了甾醇乙酸酯（SA），并表明其具有多种作用。然而，SA 的抗肥胖活性及其机制仍不清楚。
本研究在体外和斑马鱼模型中研究了从马尾藻中分离出的 SA 对 3T3-L1 脂肪细胞成脂的抑制作用。测定了 SA 处理的 3T3-L1 脂肪细胞的细胞毒性、分化、信号通路和脂质积累。从受精后 12 到 17 周测量饮食诱导肥胖（DIO）成年雄性斑马鱼的体重和甘油三酯含量。
SA 减弱了细胞的分化并减少了 3T3-L1 脂肪细胞中的脂质积累和甘油三酯含量。在脂肪细胞分化过程中，SA 抑制脂肪积累并降低了负责脂肪生成的信号因子的表达。在接受 SA 处理的脂肪细胞中，脂肪酸合成酶下调，而 AMP 激活的蛋白激酶（AMPK）上调。此外，SA 还抑制了 DIO 斑马鱼的体重和甘油三酯含量。
SA 是管理代谢紊乱（如肥胖症）的潜在治疗剂。
