Han Sun Ok, Kim Geon, Shim Youn Young, Kim Dong-Sub, Sung Nak-Yun, Nho Eun Yeong, Park Sang-Yun, Kim Hye-Lin, Kim Young Jun, Jung Yunu
Department of Food Regulatory Science, Korea University, Sejong 30019, Korea.
Division of Natural Product Research, Korea Prime Pharmacy Co., Ltd., Gyeonggi 16229, Korea.
Prev Nutr Food Sci. 2025 Aug 31;30(4):360-369. doi: 10.3746/pnf.2025.30.4.360.
Obesity is a major public health concern because of its association with metabolic disorders (e.g., type 2 diabetes mellitus) and cardiovascular diseases. Natural compounds are increasingly being explored as safer alternatives to synthetic antiobesity drugs. In this study, the antiobesity effects of extracts in 3T3-L1 adipocytes and their underlying mechanisms were investigated. Oil Red O staining was employed to assess the effects of extracts on adipogenesis and lipid accumulation, and Western blot analysis and quantitative polymerase chain reaction were used to analyze the key molecular pathways were. Treatment with extracts significantly inhibited lipid accumulation and suppressed the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, and sterol regulatory element-binding protein 1c, at the protein and mRNA levels. Furthermore, treatment with extracts activated AMP-activated protein kinase (AMPK) and enhanced the phosphorylation of acetyl-CoA carboxylase (ACC), which are key regulators of cellular energy metabolism, while reducing the total ACC expression. These findings indicate that extracts exert antiobesity effects by modulating the AMPK signaling pathway and inhibiting adipogenesis. Given these significant bioactive properties, extracts have promising application potential as a natural therapeutic agent for treating obesity.
肥胖是一个主要的公共卫生问题，因为它与代谢紊乱（如2型糖尿病）和心血管疾病有关。天然化合物正越来越多地被探索作为合成抗肥胖药物的更安全替代品。在本研究中，研究了提取物在3T3-L1脂肪细胞中的抗肥胖作用及其潜在机制。采用油红O染色评估提取物对脂肪生成和脂质积累的影响，并使用蛋白质印迹分析和定量聚合酶链反应分析关键分子途径。提取物处理在蛋白质和mRNA水平上显著抑制脂质积累，并抑制脂肪生成转录因子的表达，包括过氧化物酶体增殖物激活受体γ、CCAAT/增强子结合蛋白α和固醇调节元件结合蛋白1c。此外，提取物处理激活了AMP激活的蛋白激酶（AMPK）并增强了乙酰辅酶A羧化酶（ACC）的磷酸化，ACC是细胞能量代谢的关键调节因子，同时降低了总ACC表达。这些发现表明，提取物通过调节AMPK信号通路和抑制脂肪生成发挥抗肥胖作用。鉴于这些显著的生物活性特性，提取物作为治疗肥胖的天然治疗剂具有广阔的应用潜力。
