Almeida Cayo, Pongilio Renan Paschoalino, Móvio Marília Inês, Higa Guilherme Shigueto Vilar, Resende Rodrigo Ribeiro, Jiang Jianxiong, Kinjo Erika Reime, Kihara Alexandre Hiroaki
Laboratório de Neurogenética, Universidade Federal do ABC, São Bernardo do Campo, Brazil.
Laboratório de Sinalização Celular e Nanobiotecnologia, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Front Cell Dev Biol. 2022 Jul 4;10:926776. doi: 10.3389/fcell.2022.926776. eCollection 2022.
It is well established that temporal lobe epilepsy (TLE) is often related to oxidative stress and neuroinflammation. Both processes subserve alterations observed in epileptogenesis and ultimately involve distinct classes of cells, including astrocytes, microglia, and specific neural subtypes. For this reason, molecules associated with oxidative stress response and neuroinflammation have been proposed as potential targets for therapeutic strategies. However, these molecules can participate in distinct intracellular pathways depending on the cell type. To illustrate this, we reviewed the potential role of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2) and myeloid differentiation primary response 88 (MyD88) in astrocytes, microglia, and neurons in epileptogenesis. Furthermore, we presented approaches to study genes in different cells, employing single-cell RNA-sequencing (scRNAseq) transcriptomic analyses, transgenic technologies and viral serotypes carrying vectors with specific promoters. We discussed the importance of identifying particular roles of molecules depending on the cell type, endowing more effective therapeutic strategies to treat TLE.
颞叶癫痫（TLE）通常与氧化应激和神经炎症相关，这一点已得到充分证实。这两个过程都有助于在癫痫发生过程中观察到的改变，最终涉及不同类型的细胞，包括星形胶质细胞、小胶质细胞和特定的神经亚型。因此，与氧化应激反应和神经炎症相关的分子已被提议作为治疗策略的潜在靶点。然而，这些分子根据细胞类型可参与不同的细胞内途径。为了说明这一点，我们回顾了烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶2（NOX2）和髓样分化初级反应88（MyD88）在癫痫发生过程中在星形胶质细胞、小胶质细胞和神经元中的潜在作用。此外，我们介绍了利用单细胞RNA测序（scRNAseq）转录组分析、转基因技术和携带具有特定启动子载体的病毒血清型来研究不同细胞中基因的方法。我们讨论了根据细胞类型确定分子特定作用的重要性，这为治疗TLE提供了更有效的治疗策略。
