The Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, China.
Institute of Fungal Resources, Institute of Edible Fungus, College of Life Sciences, Guizhou University, Guiyang, China.
PeerJ. 2022 Jul 15;10:e13504. doi: 10.7717/peerj.13504. eCollection 2022.
Shifts in forest soil microbial communities over altitudinal gradients have long been attracting scientific interest. The distribution patterns of different soil microbial communities along altitudinal gradients in subtropical mountain forest ecosystems remain unclear. To better understand the changes in soil microbial communities along an altitude gradient, we used Illumina MiSeq metagenome sequencing technology to survey the soil microbial communities in a forest at four elevations (Mp1000, Mp1200, Mp1400, Mp1600) and in a tea garden in Guizhou Leigong Mountain in Southwestern China. We observed that the richness of bacteria, fungi, and viruses in the soil microbial community changed in a unimodal pattern with increasing elevation while that of Archaea first increased significantly, then decreased, and finally increased again. Euryarchaeota and Thaumarchaeota were the predominant Archaea, Proteobacteria and Acidobacteria were the predominant bacterial groups, Ascomycota and Basidiomycota were the predominant fungal groups, and Myoviridae, Podoviridae, and Siphoviridae were the predominant virus groups. Amino acid transport and metabolism, energy production and conversion, signal transduction mechanisms, and DNA replication, restructuring and repair were the predominant categories as per NOG function gene-annotation. Carbohydrate metabolism, global and overview map, amino acid metabolism, and energy metabolism were predominant categories in the KEGG pathways. Glycosyl transferase and glycoside hydrolase were predominant categories among carbohydrate enzyme-functional genes. Cluster, redundancy, and co-occurring network analyses showed obvious differences in the composition, structure, and function of different soil microbial communities along the altitudinal gradient studied. Our findings indicate that the different soil microbial communities along the altitudinal gradient have different distribution patterns, which may provide a better understanding of the mechanisms that determine microbial life in a mid-subtropical mountain forest ecosystem.
随着海拔梯度的变化，森林土壤微生物群落的变化一直引起科学界的关注。在亚热带山地森林生态系统中，不同土壤微生物群落沿海拔梯度的分布模式尚不清楚。为了更好地了解土壤微生物群落沿海拔梯度的变化，我们使用 Illumina MiSeq 宏基因组测序技术，调查了中国西南部贵州雷公山森林中四个海拔高度（Mp1000、Mp1200、Mp1400、Mp1600）的森林和茶园土壤微生物群落。我们观察到，土壤微生物群落中细菌、真菌和病毒的丰富度呈单峰模式随海拔升高而变化，而古菌的丰富度先显著增加，然后减少，最后再次增加。广古菌门和泉古菌门是主要的古菌，变形菌门和酸杆菌门是主要的细菌类群，子囊菌门和担子菌门是主要的真菌类群，肌病毒科、尾病毒科和短尾病毒科是主要的病毒类群。根据 NOG 功能基因注释，氨基酸转运和代谢、能量产生和转化、信号转导机制以及 DNA 复制、重排和修复是主要的类别。碳水化合物代谢、全局和概述图、氨基酸代谢和能量代谢是 KEGG 途径中的主要类别。糖基转移酶和糖苷水解酶是碳水化合物酶功能基因中的主要类别。聚类、冗余和共同发生网络分析显示，不同海拔梯度下土壤微生物群落的组成、结构和功能存在明显差异。我们的研究结果表明，不同海拔梯度下的土壤微生物群落具有不同的分布模式，这可能有助于更好地理解决定中亚热带山地森林生态系统中微生物生命的机制。
