Ishimoto M, Shimokawa O
Z Allg Mikrobiol. 1978;18(3):173-81. doi: 10.1002/jobm.3630180304.
E. coli was found to grow anaerobically on lactate in the presence of trimethylamine N-oxide (TMANO), reducing it to trimethylamine. Anaerobic growth on glucose was promoted in the presence of TMANO. When a culture grown in complex medium was transferred to defined medium, growth on glucose and ammonia took place in the presence of TMANO after consumption of complex nutrients introduced with the preculture, in contrast to growth in nitrate respiration. The amounts of ethanol, succinate, and lactate among the fermentation products were decreased and that of acetate was increased in the presence of TMANO. Formate generation was much reduced at pH 7.4, whereas stoichiometric formation of formate was observed in the absence of TMANO. Cells grown anaerobically in the presence of TMANO had a higher activity of amine N-oxide reductase than cells grown under other conditions. The content of cytochrome-558 was elevated in the presence of TMANO during growth in complex medium. Cytochrome c-552 found in cells grown in diluted complex medium or defined medium in the presence of TMANO was oxidized by TMANO in cell extracts. The molar growth yield on glucose was higher in the presence of TMANO than in its absence and lower than that in the presence of nitrate.
研究发现，大肠杆菌在三甲胺 N-氧化物（TMANO）存在的情况下，能够利用乳酸进行厌氧生长，并将其还原为三甲胺。在TMANO存在时，葡萄糖的厌氧生长得到促进。当将在复杂培养基中培养的菌液转移至限定培养基时，与硝酸盐呼吸条件下的生长情况不同，在消耗预培养物中引入的复杂营养物质后，在TMANO存在的情况下，菌体能利用葡萄糖和氨进行生长。在TMANO存在时，发酵产物中乙醇、琥珀酸和乳酸的含量减少，而乙酸的含量增加。在pH 7.4时，甲酸生成量大幅减少，而在没有TMANO的情况下则观察到甲酸的化学计量生成。在TMANO存在的情况下厌氧生长的细胞，其胺 N-氧化物还原酶活性高于在其他条件下生长的细胞。在复杂培养基中生长时，TMANO存在的情况下细胞色素-558的含量升高。在TMANO存在的情况下，在稀释的复杂培养基或限定培养基中生长的细胞中发现的细胞色素 c-552，在细胞提取物中会被TMANO氧化。在TMANO存在的情况下，葡萄糖的摩尔生长产量高于不存在时，但低于在硝酸盐存在的情况下。
